Macherey-Nagel™ NucleoSpin™ RNA XS Column

Purify high quality RNA quickly and easily. NucleoSpin™ RNA XS Columns enable purification of highly concentrated RNA from the smallest samples

Product Code	Brand	No. of Reactions
12703580	Macherey-Nagel™ 740902.10	10
12713580	Macherey-Nagel™ 740902.250	250
12723580	Macherey-Nagel™ 740902.50	50

Description

Features

• Isolation of RNA from small sample quantities like biopsy material or single cells
• Excellent RNA recovery and integrity
• Concentrated RNA for sensitive downstream applications by elution in as little as 5 μL
• rDNase included for on-column DNA removal
• NucleoSpin™ Filters (shredders) – efficient homogenization and reduction of viscosity
• High quality RNA, ready to use for RT-PCR and other applications
• Reducing agent TCEP – no ß-mercaptoethanol necessary

Applications

• RNA isolation from cultured cells
• RNA isolation from tissue
• RNA isolation from cryosections
• RNA isolation from laser captured cells
• RNA isolation from small amounts of plant material
• RNA isolation from samples stored in RNAlater™
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
• RNase protection assays

Specifications

• Format: Mini Spin Column
• For Use With (Application): For RNA isolation from cultured cells, tissue, cryosections and laser captured cells
• Product Type: NucleoSpin™ RNA XS Column
• Yield: 0.1 to 1.5ng (102 HeLa cells), 10 to 15ng (103 HeLa cells), 100 to 150ng (104 HeLa cells), 1000 to 1500ng (105 HeLa cells)

• Final Product Type: NucleoSpin™ RNA XS Column
• Includes: Collection tubes, NucleoSpin™ filters, collection tubes (2mL), Collection tubes (1.5mL), buffers, RNase-free rDNase, carrier RNA, reducing agent TCEP
• Quantity: 1 Each

Safety and Handling

Signal Word

• Danger

Hazard Category

• Flammable liquid Category 3
• Serious eye damage/eye irritation Category 2
• Acute toxicity Category 4
• Skin corrosion/irritation Category 2
• Respiratory sensitiser Category 1
• LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .

Supplemental information

• MIXTURE LIST-Contains : guanidinium thiocyanate, ethanol (diluted < 20 %) (denaturedwith 1%IPA/1%MEK, acc.2016/1867/EU); tr