Macherey-Nagel™ NucleoTrap™ mRNA

For Poly(A) mRNA Isolation From Total RNA

Brand:  Macherey-Nagel™ 740656

Product Code. 12708512

Description

NucleoTrap™ mRNA ensures high recovery and quality of poly(A) mRNA which is not degraded and is free of DNA contamination.
The purification process is based on affinity chromatography using latex beads and covalently modified with oligo(dT) residues. Under high-salt conditions poly(A) mRNA will be bound to the particles. The pellet of mRNA latex beads is resuspended in washing buffer and transferred to the NucleoSpin™ filter which allows an easy handling of the latex beads. After two additional washing steps poly(A) mRNA is eluted with nuclease-free water or low-salt buffer: A-dT hybrids are destabilised under lowered ionic strength conditions.NucleoTrap™ mRNA kits contain spin filters as well as all necessary reagents and buffers for isolation of highly pure poly(A) mRNA from total RNA preparations. For further analyses, enrichment of mRNA is recommended especially for construction of cDNA libraries or demanding blotting procedures in order to reduce background signals. The purified poly(A) mRNA is ready to use for all downstream applications e.g., cDNA library construction, blotting techniques, RT-PCR*, in vitro
translation, expression array and differential display.
Kit components
: NucleoTrap™ mRNA mini kit - NucleoSpin™ microfilters, microcentrifuge tubes, 24mg oligo(dT) latex beads, buffers, RNase-free water. NucleoTrap™ mRNA midi kit - NucleoSpin™ microfilters, microcentrifuge tubes, 90mg oligo(dT) latex beads, buffers, RNase-free water.

• Affinity chromotography technology
• Format: Oligo(dT) latex-bead suspension
• Sample material: 250μg total RNA (NucleoTrap mRNA mini), 1,000μg total RNA (NucleoTrap mRNA midi)
• Fragment size: 50b to 20kb
• Typical yield: 10μg mRNA (mini), 40μg mRNA (midi)
• Elution volume: 10μL to 20μL
• Binding capacity: 5μg poly(A) mRNA/20μL oligo(dT) latex bead suspension
• Preparation time: 30min/6 preps

Suitable for poly(A) mRNA isolation from total RNA; Clean-up of in vitro transcripts; Support protocol for direct purification of poly(A) mRNA from cells.

Specifications

• For Use With (Application): For Poly(A) mRNA Isolation From Total RNA
• Product Type: NucleoTrap™ mRNA
• Test Time: 30min.

• No. of Reactions: 12
• Quantity: 1 Each
• Yield: 40μg