Macherey-Nagel™ NucleoZOL

The universal reagent for RNA isolation from cells and tissue, with no chloroform or phase separation.

Brand:  Macherey-Nagel™ 740404.200

Product Code. 15443435

Description

NucleoZOL is designed for the isolation of total RNA (small and large RNA) in a single fraction or in separate fractions from a variety of sample materials.

• No chloroform, no phase separation, easy procedure
• High RNA yield and purity from any sample material
• RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms
• Small and large RNA in one or in separated fractions
• RNA ready for use in a variety of downstream applications: RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

Sample material:

• < 1 x 10⁷ cultured cells, bacteria, and yeast
• < 100 mg human/animal/plant tissue
• < 0.4 mL (viral) fluids

Typical yield:

• Total RNA
  • Liver: 6-8 μg/mg tissue
  • Kidney, spleen: 3-4 μg/mg tissue
  • Muscle, brain, lung: 0.5-1.5 μg/mg
  • Cultured cells: 4-10 μg/10⁶ cells
• Large RNA
  • Liver: 5-7 μg/mg tissue
  • Kidney, spleen: 3-4 μg/mg tissue
  • Muscle, brain, lung: 0.5-1.5 μg/mg
  • Cultured cells: 3-8 μg/10⁶ cells

Specifications

• CAS: 593-84-0; 615-004-00-3
• Content And Storage: Room temperature (18 - 25 °C)
• Sample Type: Animal/Human/Plant Tissue, Bacteria, Cultured Cells, Viral Fluids, Yeast
• Final Product Type: Total RNA
• For Use With (Application): RNA isolation from cells and tissue
• Purity: A₂₆₀/A₂₈₀: 1.8 - 2.1
• Test Time: < 1 hour

• Color: Blue
• Isolation Technology: One-phase Extraction
• Efficiency: Typical RIN: > 9
• Formulation: Phenol, Guanidinium thiocyanate, Dye
• Odor: Aromatic
• Quantity: 200 mL
• Form: Liquid

Safety and Handling

Product Identifier

• nucleozol

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 3
• Skin corrosion/irritation Category 1 B
• Germ cell mutagenicity Category 2
• Specific target organ toxicity after repeated exposure Category 2
• LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement

• H301-Toxic if swallowed.
• H311-Toxic in contact with skin.
• H314-Causes severe skin burns and eye damage.
• H331-Toxic if inhaled.
• H341-Suspected of causing genetic defects.
• H373-May cause damage to organs.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P201-Obtain special instructions before use.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P405-Store locked up.

Supplemental information

• MIXTURE LIST-Contains : phenol, guanidiniumthiocyanate