OPTIZYME™ BglI, Fisher BioReagents™

Brand:  Fisher BioReagents BP8046-1

Product Code. 11954802

Description

5'...G C C N N N N^N G G C...3'
3'...C G G N^N N N N C C G...5'

Supplied with: 10X OPTIZYME Buffer 3
Conditions for 100% Activity:

• 1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl₂, 100mM NaCl and 0.1mg/ml BSA
• Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

• Buffer 1: 0 - 20%
• Buffer 2: 50 - 100%
• Buffer 3: 100%
• Buffer 4: 0 - 20%
• Buffer 5: 100%

Storage Buffer:

• 10mM Tris-HCl (pH 7.5 at 25°C), 300mM NaCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglI.

Digestion of Agarose-embedded DNA:

A minimum of 5 units of BglI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.

Methylation Effects:

Dam: Never overlaps - no effect

Dcm: May overlap - no effect

• 5'...GCm5CW GGNNGGC...3'

3'...CG GWm5CCNNCCG...5'

(No effect on cleavage)

CpG: May overlap - cleavage impaired

• 5'...GCm5C G(N)₃m5C GGC...3'

3'...CG Gm5C(N)₃ Gm5CCG...5'

(No effect on cleavage)

• 5'...GCC(N)5GGm5C G...3'

3'...CGG(N)5CC Gm5C...5'

(Cleavage is impaired)

• 5'...m5C GCC(N)₅GGm5C G...3'

3'... Gm5CGG(N)₅CC Gm5C...5'

(Cleavage is impaired)

EcoKI: Never overlaps - no effect

EcoBI: Never overlaps - no effect

Specifications

• Concentration: 10 U/μL
• Incubator Temperature: 37°C
• For Use With (Application): >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglI
• Content And Storage: Keep container tightly closed
• Cut Site: GCCNNNN.NGGC
• Form: Liquid

• Components: 25 to 50% Water, >50% Glycerin
• pH: 7.4
• Storage Buffer: 10mM Tris HCl (pH 7.5 at 25°C), 300mM NaCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
• Quantity: 2000 U
• Product Type: BglI