OPTIZYME™ BglII, Fisher BioReagents™

Product Code	Brand	Quantity
11984852	Fisher BioReagents BP8014-5	2500 U
11964852	Fisher BioReagents BP8014-1	1000 U

Description

5'...A^G A T C T...3'
3'...T C T A G^A...5'

Supplied with: 10X OPTIZYME Buffer 3
Conditions for 100% Activity:

• 1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl₂, 100mM NaCl and 0.1mg/ml BSA
• Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

• Buffer 1: 0 - 20%
• Buffer 2: 20 - 50%
• Buffer 3: 100%
• Buffer 4: 0 - 20%
• Buffer 5: 50 - 100%

Storage Buffer:

• 10mM Tris-HCl (pH 8.2 at 25°C), 200 mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 95% of the DNA fragments can be ligated and re-cut after a 50-fold over-digestion with BglII.

Digestion of Agarose-embedded DNA:

A minimum of 5 units of BglII is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.

Compatible Ends: BamHI, BclI, Bsp143I, MboI, PsuI

Methylation Effects:

Dam: Completely overlaps - no effect

• AGm6ATCT

(No effect on cleavage)

Dcm: Never overlaps - no effect

CpG: Never overlaps - no effect

EcoKI: Never overlaps - no effect

EcoBI: May overlap - cleavage impaired

• 5'...TGm6AGATCT(N)₃ TGCT...3'

3'...AC TCTAGA(N)₃m6ACGA...5'

(Cleavage is impaired)

Specifications

• Concentration: 10 U/μL
• Incubator Temperature: 37°C
• For Use With (Application): >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglII
• Content And Storage: Keep container tightly closed
• Product Type: BglII

• Components: 25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride
• pH: 7.4
• Storage Buffer: 10mM Tris HCl (pH 8.2 at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol
• Cut Site: A.GATCT
• Form: Liquid

Safety and Handling

Product Identifier

• OPTIZYME