OPTIZYME™ BpiI (BbsI), Fisher BioReagents™

Brand:  Fisher BioReagents BP8072-1

Product Code. 11974822

Description

5'...G A A G A C (N)₂^...3'
3'...C T T C T G (N)₆^...5'

Supplied with: 10X OPTIZYME Buffer 2
Conditions for 100% Activity:

• 1X OPTIZYME Buffer 2: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl₂, 50mM NaCl and 0.1mg/ml BSA
• Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

• Buffer 1: 20 - 50%
• Buffer 2: 100%
• Buffer 3: 50 - 100%
• Buffer 4: 50 - 100%
• Buffer 5: 50 - 100%

Storage Buffer:

• 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with BpiI.

Digestion of Agarose-embedded DNA:

A minimum of 5 units of BpiI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.

Note:

• BpiI cleaves downstream of its recognition site and can generate any desired 4 base 5'-overhangs. This feature is useful for direct PCR product cloning.

Methylation Effects:

Dam: Never overlaps - no effect

Dcm: Never overlaps - no effect

CpG: May overlap - no effect

• 5'...GAAGAm5C G...3'

3'...CTTCT Gm5C...5'

(No effect on cleavage)

• 5'...m5C GAAGAC...3'

3'... Gm5CTTCTG...5'

(No effect on cleavage)

EcoKI: Never overlaps - no effect

EcoBI: May overlap - effect not determined

Specifications

• Concentration: 10 U/μL
• Incubator Temperature: 37°C
• For Use With (Application): >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BpI
• Content And Storage: Keep container tightly closed
• Cut Site: GAAGAC(2/6).
• Form: Liquid

• Components: 25 to 50% Water, >50% Glycerin
• pH: 7.4
• Storage Buffer: 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
• Quantity: 1000 U
• Product Type: BpI

Safety and Handling

Product Identifier

• OPTIZYME