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OPTIZYME™ BpiI (BbsI), Fisher BioReagents™
Brand: Fisher BioReagents BP8072-1
This item is not returnable.
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Description
5'...G A A G A C (N)2^...3'
3'...C T T C T G (N)6^...5'
Supplied with: 10X OPTIZYME Buffer 2
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 2: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/ml BSA
- Incubate at 37°C
- Buffer 1: 20 - 50%
- Buffer 2: 100%
- Buffer 3: 50 - 100%
- Buffer 4: 50 - 100%
- Buffer 5: 50 - 100%
- 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
- BpiI cleaves downstream of its recognition site and can generate any desired 4 base 5'-overhangs. This feature is useful for direct PCR product cloning.
- 5'...GAAGAm5C G...3'
- 5'...m5C GAAGAC...3'
Specifications
10 U/μL | |
37°C | |
>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BpI | |
Keep container tightly closed | |
GAAGAC(2/6). | |
Liquid |
25 to 50% Water, >50% Glycerin | |
7.4 | |
10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol | |
1000 U | |
BpI |