OPTIZYME™ HinfI, Fisher BioReagents™

Brand:  Fisher BioReagents BP8051-1

Product Code. 11994802

Description

5'...G^A N T C...3'
3'...C T N A^G...5'

Supplied with: 10X OPTIZYME Buffer 5
Conditions for 100% Activity:

• 1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl₂, 100mM KCl and 0.1mg/ml BSA
• Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

• Buffer 1: 0 - 20%
• Buffer 2: 20 - 50%
• Buffer 3: 50 - 100%
• Buffer 4: 50 - 100%
• Buffer 5: 100%

Storage Buffer:

• 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with HinfI.

Compatible Ends: G^AWTC – PfeI

Methylation Effects:

Dam: Never overlaps - no effect

Dcm: Never overlaps - no effect

CpG: May overlap - cleavage impaired

• 5'...GANTm5C G...3'

3'...CTNA Gm5C...5'

(Cleavage is impaired)

• 5'...m5C GANTm5C G...3'

3'... Gm5CTNA Gm5C...5'

(Cleavage is impaired)

EcoKI: Never overlaps - no effect

EcoBI: May overlap - blocked

• 5'...TGm6ANTC(N)₅ TGCT...3'

3'...AC TNAG(N)₅m6ACGA...5'

(Cleavage is blocked)

Specifications

• Concentration: 10 U/μL
• Incubator Temperature: 37°C
• Storage Buffer: 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
• Quantity: 2000 U
• Product Type: HinfI

• Components: 25 to 50% Water, >50% Glycerin
• For Use With (Application): >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII
• Content And Storage: Keep container tightly closed
• Cut Site: G.ANTC
• Form: Liquid

Safety and Handling

Product Identifier

• OPTIZYME