OPTIZYME™ ScaI, Fisher BioReagents™

Brand:  Fisher BioReagents BP8037-1

Product Code. 11914802

Description

5'...A G T^A C T...3'
3'...T C A^T G A...5'

Supplied with: 10X OPTIZYME AarI, ScaI, PasI Buffer
Conditions for 100% Activity:

• 1X OPTIZYME Buffer AarI, ScaI, PasI: 10mM Bis-Tris Propane-HCl (pH 6.5 at 37°C), 10mM MgCl₂ , 100mM KCl and 0.1mg/ml BSA
• Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

• Buffer 1: 0 - 20%
• Buffer 2: 0 - 20%
• Buffer 3: 0 - 20%
• Buffer 4: 0 - 20%
• Buffer 5: 0 - 20%

Storage Buffer:

• 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 80% of the DNA fragments can be ligated and more than 90% of these can be re-cut after 50-fold over-digestion with ScaI.

Digestion of Agarose-embedded DNA:

A minimum of 20 units of ScaI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.

Notes:

• Conditions of low salt, high enzyme concentration, glycerol concentration >5% or pH >8.0 may result in star activity.
• Supercoiled plasmids may require up to 20-fold more ScaI for complete digestion than linear DNA.

Methylation Effects:
Dam: Never overlaps - no effect
Dcm: Never overlaps - no effect
CpG: Never overlaps - no effect
EcoKI: Never overlaps - no effect
EcoBI: May overlap - blocked

• 5'...TGm6AGTACT(N)₃ TGCT...3'

3'...AC TCATGA(N)₃ m6ACGA...5'

(Cleavage blocked)

Specifications

• Concentration: 10 U/μL
• Incubator Temperature: 37°C
• For Use With (Application): >80% of DNA fragments can be ligated and >90% of these can be re-cut after 50-fold over-digestion with ScaI
• Quantity: 1000 U
• Product Type: ScaI

• Components: 10X OPTIZYME™ AarI, ScaI, PasI Buffer
• pH: 7.4
• Storage Buffer: 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol
• Cut Site: AGT.ACT
• Form: Liquid

Safety and Handling

Product Identifier

• OPTIZYME