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OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody (eBio25-D1.16 (25-D1.16)), APC, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Invitrogen 17-5743-80
Description
Description: The 25-D1.16 monoclonal antibody reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb, or H-2Kb bound with an irrelevant peptide. This antibody has proven to be very useful tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo. Applications Reported: This eBio25-D1.16 (25-D1.16) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio25-D1.16 (25-D1.16) antibody has been tested by flow cytometric analysis of SIINFEKL peptide pulsed mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Cells can be pulsed with the SIINFEKL peptide according to the following protocol: 1. With cells in flow staining buffer, add SIINFEKL peptide to a final concentration of 30 uM. 2. Incubate cells at 37°C for 2 hours. 3. Wash cells with flow staining buffer. 4. Proceed with cell surface staining as normal. For additional information see the references listed below. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.
OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal antibody specifically detects OVA257-264 (SIINFEKL) peptide bound to H-2Kb in Mouse samples. It is validated for Flow Cytometry.Specifications
OVA257-264 (SIINFEKL) peptide bound to H-2Kb | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
Mouse | |
25 μg | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
eBio25-D1.16 (25-D1.16) | |
APC | |
H-2Kb-SIINFEKL; OVA257-264; OVA257-264 H-2Kb; OVA-Kb | |
Affinity chromatography | |
RUO | |
Mouse | |
Antibody | |
IgG1 κ |
For Research Use Only.