Thermo Scientific™ Escherichia coli O157 Latex Test

Rapidly confirm E. coli serogroup O157 from colonies cultured on plated media by latex agglutination.

Brand:  Thermo Scientific™ DR0620M

Product Code. 11409888

Description

A latex agglutination test for the identification of E. coli serogroup O157. Certain strains of Escherichia coli have been implicated in cases of haemorrhagic colitis (HC) and haemolytic ureamic syndrome (HUS). It has been shown that these strains produce a vero-cytotoxin (VT). The E coli serotype most frequently isolated from HC and HUScases is O157:H7. Isolation of this serotype from a diarrhoeal stool particularly with the presence of blood is indicative of a vero-cytotoxin producing strain^{1, 2, 3, 4, 5, 6, 7 ,8}. The Oxoid E coli O157 latex test will demonstrate by slide agglutination E. coli strains possessing the O157 serogroup antigen. The test is best used in conjunction with Sorbitol MacConkey Agar (Oxoid CM0813). E. coli O157:H7 strains do not ferment Sorbitol and therefore give colourless colonies on this medium. The majority of E coli isolates do ferment Sorbitol and give characteristic pink colonies. Sorbitol MacConkey Agar should be used as the primary screen. Non-sorbitol fermenting colonies can then be tested with the latex reagents, to determine whether the isolate belongs to the O157 serogroup and therefore a potential VT- producing strain.

Use this dry latex test designed to demonstrate by slide agglutination E. coli strains possessing the O157 serogroup antigen.

The test is best used in conjunction with Sorbitol MacConkey Agar. E. coli O157:H7 strains do not ferment sorbitol and, therefore, give colorless colonies on this medium. The majority of E. coli isolates do ferment sorbitol and give characteristic pink colonies. Sorbitol MacConkey Agar should be used as the primary screen. Non-sorbitol fermenting colonies can then be tested with the latex reagents to determine whether the isolate belongs to the O157 serogroup and, therefore, a potential verotoxin-producing strain.

• Pre-dispensed reagents eliminate extra steps for faster time to result
• Room temperature storage for up to two years simplifies inventory
• Blue latex particles are sensitized with reactive (positive) and non-reactive (negative) antibodies and dried onto reaction cards for convenience

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand, combining powerful manual, semi-automated and fully automated test products and a comprehensive line of media and diagnostic products to offer a complete, end-to-end solution to quickly deliver the products you need and the quality results your laboratory depends on.

Specifications

• Content And Storage: 2°C to 8°C
• Detectable Analytes: E. coli
• Type: E. coli Testing Kit

• Description: E. coli O157 Latex Test
• Sample Type: E. coli Colonies from Plate
• Quantity: 100 Tests/Kit