p27 Kip1 Monoclonal Antibody (DCS-72.F6), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen AHZ0452

Product Code. 17164759

Description

Suggested positive controls include HT29, MAD109, PC12 cells and brain tissue.

p27 Kip1 functions as a negative regulator of G1 mouse expression and has been proposed to function as a possible mediator of TGFb induced G1 arrest. p27 Kip1 is an important regulator of cell cycle progression, a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. p27 Kip1 acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. p27 Kip1 has a molecular weight of approximately 27kDa and the degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Specifications

• Antigen: p27 Kip1
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Gene: CDKN1B
• Gene Alias: CDKN1B, CDN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, KIP1
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 1027, 12576, 403429, 83571
• Content And Storage: 4° C
• Form: Liquid

• Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Clone: DCS-72.F6
• Conjugate: Unconjugated
• Gene Accession No.: Q6SLL5, P46527, P46414
• Gene Symbols: CDKN1B
• Immunogen: Mouse recombit p27Kip1 protein.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Canine, Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1 κ