Lonza™ P3 Primary Cell 4D-Nucleofector™ X Kit S

For efficient transfection of specific primary cells, e.g., human T cells, CD34 or rat neurons, in the 4D-NucleofectorTM X Unit (20 μL format).

Brand:  Lonza™ V4XP-3032

Product Code. 12618553

Description

• High transfection efficiencies for primary cells.
• Cells maintain functionality post transfection.
• Conditions are transferable between 4D-Nucleofector™ System, 96-well Shuttle™ System and 384-well Nucleofector™ System.
• Applications:
  • Transfection of cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cells.
  • Medium throughput applications.
  • Optimization of transfection conditions, e.g., substrate titrations.
• This kit has been determined to be optimal for transfecting following cell types:
  • B cell (human)
  • CD34+ cell (human)
  • Chondrocyte (human)
  • DC (human)
  • DC (mouse), mature
  • ES (human), H9
  • ES (mouse)
  • Hepatocyte (human)
  • HMEC (human)
  • Keratinocyte (human)
  • Macrophage (human)
  • Monocyte (human)
  • Neuron (rat), brain Hi/Cx
  • NHBE (human)
  • RPE (human)
  • T cell (human), stim.
  • T cell (mouse), unstim.

Specifications

• Color: Clear
• For Use With (Equipment): 4D-Nucleofector™ X Unit (20 μL format)
• Includes: P3 Primary Cell Nucleofector™ Solution, Supplement 1, pmaxGFP™ Vector (1 μg/μL in 10 mM Tris pH 8.0), 16-well Nucleocuvette™ Strips
• pH: 7.1 to 7.3

• Vector: pmaxGFP™
• For Use With (Application): Efficient transfection of specific primary cells, e.g., human T cells, CD34 or rat neurons.
• No. of Reactions: 32
• Quantity: 2 x 0.675 mL P3 Primary Cell Nucleofector™ Solution, 2 x 0.15 mL Supplement 1, 50 μg pmaxGFP™ Vector (1 μg/μL in 10 mM Tris pH 8.0), 2 x 16-well Nucleocuvette™ Strips