p38 MAPK Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen OPA110080

Product Code. 11530303

Description

OPA1-10080 detects p38 from human, mouse and rat samples. OPA1-10080 has been successfully used in Western blot, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ∽38 kDa protein representing p38 from HeLa whole cell lysates. OPA1-10080 immunizing peptide corresponds to the carboxy terminal region of mouse p38.

The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.

Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly

Specifications

• Antigen: p38 MAPK
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MAPK14
• Gene Alias: Crk1; CSAID-binding protein; Csaids binding protein; CSBP; CSBP1; CSBP2; CSPB1; cytokine suppressive anti-inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug binding protein 1; cytokine suppressive anti-inflammatory drug-binding protein; cytokine-supressive anti-inflammatory drug binding protein; Exip; Hog; MAP kinase 11; MAP kinase 13; MAP kinase 14; MAP kinase 2; MAP kinase Mxi2; MAP kinase p38 alpha; MAP kinase p38 beta; MAP kinase p38 delta; MAPK 11; MAPK 13; MAPK 14; Mapk11; Mapk13; MAPK-13; Mapk14; MAX-interacting protein 2; mitogen activated protein kinase 11; mitogen activated protein kinase 13; mitogen activated protein kinase 14; mitogen-activated protein kinase 11; Mitogen-activated protein kinase 13; mitogen-activated protein kinase 14; mitogen-activated protein kinase 14A; mitogen-activated protein kinase p38 alpha; Mitogen-activated protein kinase p38 beta; mitogen-activated protein kinase p38 delta; mitogen-activated protein kinase p38-2; MXI2;
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1432, 26416, 81649
• Content And Storage: 4° C, do not freeze
• Form: Liquid

• Applications: Immunocytochemistry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.2% gelatin and 0.1% sodium azide
• Gene Accession No.: P47811, P70618, Q16539
• Gene Symbols: MAPK14
• Immunogen: Synthetic peptide corresponding to the carboxy terminal region of mouse p38.
• Quantity: 1 mL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG