Molecular Probes™ pHrodo™ Red Zymosan BioParticles™ Conjugate for Phagocytosis

Study endocytosis and phagocytosis regulation by drugs and/or environmental factors with the pHrodo Red Zymosan Bioparticles Conjugate for Phagocytosis, which contains pHrodo Red indicator dye and is compatible with HCS, HTS, fluorescence microplate readers, and flow cytometry.

Brand:  Molecular Probes™ P35364

Product Code. 13355464

Description

Achieve faster staining and more accurate results without the need for wash steps or quencher dye with pH-sensitive pHrodo Red Zymosan BioParticles Conjugate for Phagocytosis. This dye-bioparticle conjugate provides faster and more accurate results than any other dye-bioparticle conjugate and has reduced signal variability and improved timing in sensitive experiments. pHrodo Red dye conjugates are non-fluorescent outside the cell, but fluoresce bright red in phagosomes, specifically detecting intracellular phagocytosis and endocytosis. The pHrodo Red Zymosan BioParticles Conjugate can also be multiplexed with a wide variety of blue, green, and far-red dyes and reporters such as GFP, Fuo-4, calcein, NucBlue, CellEvent Caspase 3/7 green, Mitosox Green, and Mitotracker Deep Red, among many others.

The fluorescence of the pHrodo Red dye dramatically increases as pH decreases from neutral to acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. Its lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes.

Use the ready-made pHrodo Red Zymosan BioParticles Conjugate in imaging or flow applications, or pHrodo Red SE, the activated succinimidyl ester, for labeling microorganisms or proteins of your choice.

In addition, pHrodo dye is available in the color green, and with E. coli, zymosan, S. aureus, and dextran 10,000 MW conjugates.

Specifications

• Color: Red
• Description: pHrodo™ Red Zymosan BioParticles™ Conjugate for Phagocytosis
• For Use With (Application): Cell Analysis
• Product Type: Dye
• Product Line: pHrodo™
• Shipping Condition: Room Temperature
• Excitation/Emission: 560/585

• Content And Storage: Store at –20°C, desiccate, and protect from light.
  
  
• Detection Method: Fluorescence
• For Use With (Equipment): Attune Acoustic Focusing Cytometer, Confocal Microscope, Floid Cell Imaging System, Fluorescence Microscope, High Content Analysis Instrument, Flow Cytometer
• Dye Type: pH-Sensitive Dyes
• Quantity: 5 x 1 mg
• Species: S. cerevisiae

For Research Use Only. Not for human or animal therapeutic or diagnostic use.