Thermo Scientific™ Phusion™ Hot Start II DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Includes: Phusion Hot Start II High–Fidelity DNA Polymerase

• Phusion Hot Start II High–Fidelity DNA Polymerase (2U/μL)
• 5x Phusion HF Buffer
• 5x Phusion GC Buffer
• DMSO
• 50mM MgCl₂ solution

Phusion Green Hot Start II High–Fidelity DNA Polymerase

• Phusion Hot Start II High–Fidelity DNA Polymerase (2U/Glc muL)
• 5x Phusion Green HF Buffer
• 5x Phusion Green GC Buffer
• DMSO
• 50mM MgCl2 solution

Both Phusion and Phusion Green Buffer provide 1.5 mM MgCl₂ in the final 1x concentration

Product Code	Brand	Color	No. of Reactions
13505170	Thermo Scientific™ F537S	Green	100 Reactions
13595160	Thermo Scientific™ F537L	Green	500 Reactions
10441338	Thermo Scientific™ F549S	Colorless	100 Reactions
10628439	Thermo Scientific™ F549L	Colorless	500 Reactions
16258310	Thermo Scientific™ F549XL	Colorless	2000 Reactions

Description

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

With Phusion Hot Start II High-Fidelity DNA Polymerase amplification proceeds without the production of nonspecific products due to the combination of Phusion DNA Polymerase and a reversibly bound, specific Affibody ligand that inhibits DNA polymerase activity at room temperature. The Affibody ligand also inhibits the 3' to 5' exonuclease activity of the polymerase, thus preventing degradation of primers and template DNA during reaction set up. At temperatures that promote polymerase activity, the ligand is released, rendering the polymerase fully active. Phusion Hot Start II DNA Polymerase is immediately reactivated at high temperatures, so it does not require a separate activation step in PCR protocols.

Phusion Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion Hot Start II DNA Polymerase and the 5X Phusion buffers. The buffers include a density reagent and two tracking dyes which do not interfere with the robust performance of Phusion Hot Start II DNA Polymerase. Researchers may directly load PCR products on a gel making this a versatile option for use in downstream applications such as DNA sequencing, ligation and restriction digestion.

Highlights

• Reaction set up at room temperature
• No non-specific amplification and primer degradation during reaction set up
• Zero-time reactivation due to unique hot start technology
• High fidelity (52X Taq)
• Fast PCR due to short extension times (15–30 s/kb)
• Robust reactions, minimal optimization needed
• Increased product yields with minimal enzyme amounts
• Direct loading on gels

Applications

• High-fidelity PCR
• High throughput
• Amplification of difficult (GC-rich) templates
• Template generation for sequencing
• Multiplex PCR
• Long-range PCR
• Cloning
• Mutagenesis
• Microarray

Using Phusion DNA polymerases

Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).

Specifications

• GC-Rich PCR Performance: High
• Shipping Condition: Dry Ice
• Concentration: 2 U/μL
• Hot Start: Built-In Hot Start
• Reaction Format: Standalone

• Reaction Speed: Fast
• For Use With (Application): Hot-start PCR, High-fidelity PCR
• Fidelity (vs. Taq): 52X
• Overhang: Blunt
• Size (Final Product): 20 kb or less

For Research Use Only. Not for use in diagnostic procedures.