Applied Biosystems™ PicoPure™ RNA Isolation Kit

PicoPure™ RNA Isolation Kit

Product Code	Brand	No. of Samples
15295033	Applied Biosystems™ KIT0204	40 samples

Description

The ARCTURUS™ PicoPure™ RNA Isolation Kit is designed to recover high-quality total RNA consistently from fewer than ten cells, even from a single cell. This kit is designed to provide consistent, efficient RNA recovery without sacrificing quality. Small volume elution allows you to maximize your recovery of RNA from small numbers of cells for use with your gene expression analysis.

Key Product Features:

Greater sensitivty—isolate RNA from even a single cell

Superior efficiency—maximize RNA recovery from samples

Streamlined concentration—elute with small volumes

Flexible application—compatible with many tube sizes

Integrated contamination prevention—column lids ensure that samples remain RNase free

Isolate RNA from Even a Single Cell
The PicoPure™ RNA Isolation Kit is engineered to recover high-quality total RNA consistently from fewer than ten cells. The high recovery rate attained using this kit is valuable not only for small samples containing picograms of RNA but also for samples containing up to 100μg of RNA. Since the RNA elutes in as little as 10μl of buffer, it is ready for gene expression analysis without any vacuum concentration. This kit is available in two sizes, enough for 40 or 200 isolations.

Get Superior RNA Recovery
Small, valuable samples often cannot be replaced. Performing gene expression studies on these precious samples requires getting maximum RNA recovery. Comparative studies show that, with small, RNA-poor cell or tissue samples, the PicoPure™ Kit dramatically outperforms other commercial total RNA isolation kits in efficiency.

Eliminate Time-consuming RNA Concentration
The simple isolation protocol consists of extracting cellular RNA, then loading the extract onto the specially designed MiraCol™ Purification Column to bind the RNA. After washing away impurities, the RNA elutes in only 10μl of buffer, ready for use without vacuum concentration or transfer into a new reaction tube.

High Yield Purification Columns
The PicoPure™ RNA Isolation Kit features unique, high-recovery MiraCol™ Purification Columns, engineered to maximize recovery from microscopic samples. The columns have convenient integrated lids to help ensure that samples remain RNase free. In addition, the Kit comes complete with all necessary buffers, an easy-to-follow User Guide, optimized protocols for handling Laser Capture Microdissected (LCM) samples, and protocols for isolating RNA from larger samples.

Deliver RNA Ready for Amplification and Microarray Analysis
RNA isolated with the PicoPure™ RNA Kit is ready, without vacuum concentration, for amplification with the RiboAmp™ RNA Amplification Kit. The two kits together allow for highly reproducible gene expression results.

Retain Low-abundance mRNA
The PicoPure™ RNA Isolation Kit preserves mRNA in all abundance classes through the isolation process, minimizing the loss of important genes of interest. Low, medium, and high-abundance genes are consistently recovered and available for expression analysis.

Maintain High RNA Quality
The PicoPure™ RNA isolation process allows for high-quality RNA. Total cellular RNA prepared from LCM samples remains intact after isolation, increasing confidence in subsequent molecular analysis.

Specifications

• Content And Storage: Extraction buffer, purification reagents, purification columns, microcentrifuge tubes. Store at room temperature
• Isolation Technology: Spin Column
• Final Product Type: Total RNA
• For Use With (Equipment): ArcturusXT™ LCM Instrument
• Product Line: PicoPure™
• Target: Total RNA

• Format: Spin Column
• Sample Type: Laser Capture Microdissection (LCM), Tissue (Frozen)
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• For Use With (Application): Microarray Analysis, Real-Time Quantitative PCR (qPCR)
• Starting Material Amount: As little as a single cell.

Safety and Handling

Product Identifier

• RNA Isolation Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Serious eye damage/eye irritation Category 2
• Flammable liquid Category 2
• Flammable liquid Category 3
• Skin corrosion/irritation Category 1 C
• Skin corrosion/irritation Category 2
• Skin sensitiser Category 1

Hazard Statement

• H225-Highly flammable liquid and vapour.
• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H314-Causes severe skin burns and eye damage.
• H315-Causes skin irritation.
• H317-May cause an allergic skin reaction.
• H319-Causes serious eye irritation.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P233-Keep container tightly closed.
• P242-Use only non-sparking tools.
• P243-Take precautionary measures against static discharge.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P330-Rinse mouth.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P370+P378-In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
• P403+P235-Store in a well-ventilated place. Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : 2-Mercaptoethanol, Thiocyanic acid, ethanol

For Research Use Only. Not for use in diagnostic procedures.