Thermo Scientific™ 1-Step™ ABTS Substrate Solution

Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.

Brand:  Thermo Scientific™ 37615

Product Code. 10269423

Description

Thermo Scientific™ Pierce ABTS Substrates are tablets and a ready-to-use solution of a water-soluble peroxidase substrate that yields a measurable green end product for use in ELISA methods.

ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt) is a water-soluble HRP substrate that yields a green end product upon reaction with peroxidase. The green product has two major absorbance peaks, 410nm and 650nm. ABTS is less sensitive than OPD and TMB in ELISA applications. It is less readily oxidized, and its color development is slower (approximately 20 minutes). This may be advantageous if unacceptable background results from the use of the OPD or TMB substrates due to higher sensitivities.

Highlights:

• Excellent choice when maximum sensitivities are not required
• Less readily oxidized and color development is slower (approximately 20 minutes) than OPD and TMB

Specifications

• Description: Pierce 1-Step ABTS Solution
• Detection Method: Colorimetric
• Substrate Properties: Chemical Substrate
• Content And Storage: Store at 4°C

• Product Type: ELISA Substrate
• Product Line: 1-Step™
• Substrate Type: HRP (Horseradish Peroxidase)

Safety and Handling

Product Identifier

• ABTS Substrate Solution

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 1
• Skin corrosion/irritation Category 2
• Specific target organ toxicity after repeated exposure Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H318-Causes serious eye damage.
• H373-May cause damage to organs.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : 2,2'-iminodiethanol,sodium azide

For Research Use Only. Not for use in diagnostic procedures.