Thermo Scientific™ Pierce™ Microplate BCA Protein Assay Kit - Reducing Agent Compatible

Reducing agent-compatible version of our popular BCA Reagent Kit to measure protein concentration in samples containing DTT or BME thiol-reductants.

Brand:  Thermo Scientific™ 23252

Product Code. 10209523

Description

This reducing agent-compatible (RAC) version of the Thermo Scientific™ Pierce BCA Protein Assay provides accurate determination of protein concentration in samples that contain dithiothreitol (DTT), 2-mercaptoethanol (BME), TCEP and other disulfide reducing agents.

The BCA Protein Assay Kit - Reducing Agent Compatible (BCA-RAC) provides all of the advantages of the original BCA Assay, plus compatibility with disulfide reducing agents at concentrations routinely used in protein sample buffers. This special adaptation of the popular Pierce BCA Protein Assay method enables accurate protein concentration measurement for samples containing DTT, 2-ME or TCEP. Available in tube and microplate formats, these reducing agent compatible (RAC) BCA Kits extend the already broad reagent compatibility of the BCA Protein Assay protocol to include nearly all types of components commonly present in protein research samples.

Highlights:

• Compatible – assay samples that contain up to 5mM DTT, 35mM BME, or 10mM TCEP

• BCA technology – only a slight modification of the standard BCA Protein Assay protocol (15-minute incubation with Compatibility Reagent); no precipitation steps required

• Small samples – requires only 25μL (standard kit) or less than 10μL (microplate kit) of sample

• Colorimetric – measure with a standard spectrophotometer or plate reader (562nm)

• Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods

• High linearity – linear working range for BSA equals 125 to 2000μg/mL

Includes:

BCA Reagents A and B, Compatibility Reagent, Reconstitution Buffer, and Albumin Standard (2mg/mL); Microplate kit also includes assay-compatible 96-well microplates

Specifications

• Content And Storage: Sufficient For: 980 microplate assays with 9 μL samples
  • BCA Reagent A, 250 mL
  • BCA Reagent B, 25 mL
  • Compatibility Reagent, 48 x 10 mg
  • Reconstitution Buffer, 15 mL
  • Albumin Standard Ampules, 2mg/mL, 10 x 1 mL
  • 96-Well Microplates, 20 plates
  
  Store at room temperature
• Assay: BCA Assay
• Product Line: Pierce™
• Specificity: Not Target-Specific
• For Use With (Application): Solution-based Detection, Absorbance
• Quantity: 275 mL

• Description: Pierce BCA Microplate Protein Assay Kit, Reducing Agent Compatible (RAC)
• Detection Method: Colorimetric
• Product Type: Protein Quantitation Assay
• Sufficient For: 980 Microplate Assays
• For Use With (Equipment): Spectrophotometer, Microplate Reader

Safety and Handling

Product Identifier

• Pierce Microplate BCA Protein Assay Kit - Reducing Agent Compatible

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 3
• Respiratory sensitiser Category 1
• Skin sensitiser Category 1
• ACUTE AQUATIC Acute 1
• LONG-TERM AQUATIC HAZARD Chronic 2

Hazard Statement

• H301-Toxic if swallowed.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H317-May cause an allergic skin reaction.
• H400-Very toxic to aquatic life.
• H411-Toxic to aquatic life with long lasting effects.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P284-¢In case of inadequate ventilation! wear respiratory protection.
• P273-Avoid release to the environment.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P391-Collect spillage.
• P302+P352-IF ON SKIN: Wash with plenty of water/.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P405-Store locked up.
• P501-Dispose of contents/container to .

Supplemental information

• MIXTURE LIST-Contains : sodium carbonate, ¢2, 2'-Biquinoline!-4,4'-dicarboxylic acid, sodium salt (1:2), Sulfuric acid copp

For Research Use Only. Not for use in diagnostic procedures.