Thermo Scientific™ Pierce™ Bradford Protein Assay Kit

Brown-to-purple (A595nm), ready-to-use, classic reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.

Brand:  Thermo Scientific™ 23200

Product Code. 10270014

Description

Measure protein concentration using a stable formulation of the original Bradford reagent described in the literature with Thermo Scientific™ Pierce Coomassie Protein Assay Kit.

The Pierce Coomassie Protein Assay Kit is a ready-to-use formulation of the popular assay reagent originally described by Bradford in 1976. When mixed with a protein solution, the acidic Coomassie-dye reagent changes color from brown to blue in proportion to the amount of protein present in the sample. Protein determinations are made by comparison to the color response of protein assay standards, usually prepared as a series of known dilutions of bovine serum albumin (BSA) or bovine gamma globulin (BGG). The kit includes Coomassie Protein Assay Reagent and a package of Albumin Standard Ampules. The simple procedure is adaptable to nearly any volume scale, including test tubes, cuvets and microplates.

Highlights:

• Bradford reagent – stable, ready-to-use kit of the classical Bradford assay reagent

• Colorimetric – measure with a standard spectrophotometer or plate reader (595nm)

• Easy to use – single reagent; no working reagent preparation required

• Fast – almost immediate color development; add, mix and read results

• Broad range – detects protein concentration in the range 1 to 1500μg/mL

• Flexible – microplate and cuvet protocols provided with the instructions and adaptable to several target working ranges

Includes:

Coomassie Protein Assay Reagent and 10 ampules of Albumin Standard

Specifications

• Content And Storage: Sufficient For:
  • 630 tube assays or 3800 microplate assays
  • Pierce Bradford Assay Reagent, 950 mL
  • Albumin Standard ampules, 2 mg/mL, 10 x 1 mL
  Store Bradford Protein Assay Reagent at 4°C. Store unopened Albumin Standard ampules at room temperature.
• Detection Method: Colorimetric
• Product Type: Protein Quantitation Assay
• Sufficient For: 630 Tube Assays or 3800 Microplate Assays
• For Use With (Equipment): Spectrophotometer, Microplate Reader

• Assay: Bradford Assay
• Product Line: Pierce™
• Specificity: Not Target-Specific
• For Use With (Application): Solution-based Detection, Absorbance
• Quantity: 950 mL

Safety and Handling

Product Identifier

• Pierce Coomassie (Bradford) Protein Assay Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• Skin corrosion/irritation Category 1 B
• Serious eye damage/eye irritation Category 1
• Specific target organ toxicity Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H312-Harmful in contact with skin.
• H332-Harmful if inhaled.
• H314-Causes severe skin burns and eye damage.
• H318-Causes serious eye damage.
• H371-May cause damage to organs.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P331-Do NOT induce vomiting.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305-IF IN EYES:.
• P405-Store locked up.
• P501-Dispose of contents/container to .

Supplemental information

• MIXTURE LIST-Contains : phosphoric acid, methanol

For Research Use Only. Not for use in diagnostic procedures.