Thermo Scientific™ Pierce™ Cypridina Luciferase Glow Assay Kit

Obtain bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.

Brand:  Thermo Scientific™ 16171

Product Code. 12201632

Description

The Thermo Scientific™ Pierce Cypridina Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.

This Pierce Luciferase Glow Assay Kit contains reagents for measuring Cypridina luciferase activity in mammalian cell culture media and lysates. When used with Thermo Scientific Cypridina Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of luciferase activity. The light output generated by the luciferase reaction can be correlated to the amount of luciferase protein produced, which in turn is proportional to the activity of the promoter driving the luciferase expression. The kit can be used to detect the activity of any Cypridina luciferases or derivatives that use Vargulin as a substrate.

Highlights:

• Sensitive – highly sensitive detection of Cypridina luciferase activity

• Secreted luciferase – allows real-time assays and kinetic studies without destroying cells

• Stable – reagents provide increased signal stability compared to flash assays

• Simple – does not require a luminometer with injectors

• Compatible – assay reagents compatible with other Cypridina or Vargula luciferases

• Automation-friendly – amenable to high throughput assays

• Convenient – contains a universal cell lysis buffer and optimized glow assay reagent

• Safe – allows one to perform non-radioactive assays

Includes:

Cell lysis buffer, reaction buffer and substrate

Requires:

Cypridina luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers

Recommended for:

Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Secretory pathway / protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies

Specifications

• Type: Assay Kit
• Quantity: 1000 Reaction Kit
• Assay: Reporter Enzyme, Luciferase Reporter Assay
• Detection Method: Bioluminescence
• Format: 384-well plate, 96-well plate
• Substrate Properties: Chemical Substrate
• Substrate Type: Luciferase Substrate
• Substrate: Vargulin (Cypridina Luciferin)

• Target: Luciferase, Cypridina Luciferase
• Content And Storage: • Cypridina Glow Assay Buffer, 5 mL, store at 4°C
  • Vargulin (1X), 0.5 mL, store at -20°C
  • 2X Cell Lysis Buffer, 6 mL, store at room temperature
  
  Upon receipt store kit at -20°C or store individual components as indicated above.
• Compatible Cells: Mammalian Cells
• For Use With (Equipment): Luminometer (Microplate)
• Label Type: Enzyme Labeled
• Technique: Enhanced Chemiluminescence
• Product Line: Pierce™

For Research Use Only. Not for use in diagnostic procedures.