Thermo Scientific™ Pierce™ Fast Western Blot Kit, ECL Substrate

Optimized reagents that shorten the time to perform a typical Western blot from 4 hours to approximately 55 minutes.

Brand:  Thermo Scientific™ 35050

Product Code. 10510024

Description

Thermo Scientific™ Pierce Fast Western Blot Kit, ECL Substrate contains optimized reagents that shorten the time to perform a typical Western blot from 4 hours down to approximately 55 minutes.

The kit provides all the reagents necessary to complete a Western blot being probed with a mouse or rabbit primary antibody. The protocol requires minimal hands-on time and yields results comparable to classic Western blotting with ECL. The included Pierce ECL Substrate produces a chemiluminescent signal, which is detected using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the primary antibody and immunodetection reagents and reprobed.

Highlights:

• Fast – all the sensitivity of Pierce ECL substrate; saves three to four hours over traditional blotting methods

• Convenient – no expensive hardware or extra bench space needed; eliminates membrane clogging issues

• Economical – costs about the same as a classical Western blot; no expensive consumables or extra equipment

• Easy – optimized kits includes all reagents except primary antibody

• Versatile – kits work with both rabbit and mouse primary antibodies

Includes:

Kits contain all reagents to perform Western blots probed with mouse and rabbit primary antibodies, including: Fast Western Antibody Diluent; Fast Western 10X Wash Buffer; Fast Western Optimized HRP Reagent; Pierce ECL Detection Reagent 1; Pierce ECL Detection Reagent 2

Specifications

• Product Type: Western Blot Kit
• Form: Liquid
• Membrane Compatibility: Nitrocellulose, PVDF
• Quantity: 1 L Kit
• Substrate: Horseradish Peroxidase
• Sufficient For: 25 Mini Blots

• Detection Method: Chemiluminescence
• For Use With (Application): Western Blot
• Product Line: Pierce™
• Conjugate: HRP
• Reactivity: Mouse, Rabbit
• Content And Storage: Sufficient For: 25 mini blots probed with mouse or rabbit primary antibodies
  • Fast Western Antibody Diluent, 500 mL
  • Fast Western 10X Wash Buffer, 250 mL
  • Fast Western Optimized HRP Reagent, 25 mL
  • Pierce ECL Detection Reagent 1, 125 mL
  • Pierce ECL Detection Reagent 2, 125 mL
  
  Store at 4°C.

Safety and Handling

Product Identifier

• Fast Western Blot Kit, ECL Substrate

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Skin sensitiser Category 1

Hazard Statement

• H315-Causes skin irritation.
• H317-May cause an allergic skin reaction.
• H319-Causes serious eye irritation.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P272-Contaminated work clothing should not be allowed out of the workplace.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Sodium chloride, Tris (hydroxymethyl) aminomethane, Nonidet

For Research Use Only. Not for use in diagnostic procedures.