Thermo Scientific™ Pierce™ Fe-NTA Phosphopeptide Enrichment Kit

Purify phosphorylated peptides to process protein digests or cation-exchange fractions for mass spectrometry using spin columns of iron-chelate agarose resin.

Brand:  Thermo Scientific™ 88300

Product Code. 10389287

Description

Thermo Scientific™ Pierce Fe-NTA Phosphopeptide Enrichment Kit enables fast and efficient enrichment of phosphorylated peptides to process protein digests or strong cation-exchange peptide fractions for analysis by mass spectrometry.

The Pierce Fe-NTA Phosphopeptide Enrichment Kit enriches phosphopeptides from complex samples using iron-chelate resin in spin columns. These columns enrich a higher percentage of phosphopeptides than other resins and with an overall higher number of total and unique phosphopeptides. The complete kit is easy to use and requires less than one hour to process protein digests or strong cation-exchange peptide fractions for analysis by mass spectrometry (MS).

Highlights:

• Convenient — spin-column format enables parallel processing of multiple samples
• High capacity — each column enriches up to 150μg of phosphopeptides
• Excellent recovery — our kit enriches more total and unique phosphopeptides than other commercially available resins
• High coverage — recover peptides with single and multiple phosphorylation sites

Product Details:

To achieve robust MS results, enrichment of phosphopeptide samples is essential because of low abundance and poor ionization relative to non-phosphorylated peptides. Multiple phosphorylated amino acids within a peptide contribute to the complexity of phosphopeptide analysis. The Pierce Fe-NTA Spin Columns effectively enriches singly phosphorylated peptides as well as peptides with three or more phosphorylated sites, reducing sample complexity and enabling effective identification and characterization of phosphorylated peptides by MS. The Pierce Fe-NTA Phosphopeptide Enrichment Kit contains detailed instructions and all necessary components to load, wash and elute phosphopeptides within an hour. This kit is compatible with samples digested in solution or after in-gel digestion. Using the Pierce Graphite Spin Columns (Part No. 88302) after enrichment will improve phosphopeptide analysis by efficiently binding hydrophilic peptides and efficiently removing urea, salts and other contaminants before MS analysis.

Specifications

• Description: Pierce Fe-NTA Phosphopeptide Enrichment Kit
• Kit Contents: Binding Buffer, 6mL, Elution Buffer, 6mL, Fe-NTA Spin Columns, 30 columns, Wash Buffer (2X), 12mL
• Quantity: 30 Column Kit

• Formulation: Multi-component kit with Fe-NTA Spin Columns
• Sufficient For: Processing 30 samples (200µL each) containing up to 150µg of phosphopeptides each
• For Use With (Application): MALDI-MS