Thermo Scientific™ Pierce™ Firefly Luciferase Glow Assay Kit

Obtain bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of red firefly luciferase reporters

Product Code	Brand	Quantity
12112250	Thermo Scientific™ 16176	100 Reaction Kit
12122250	Thermo Scientific™ 16177	1000 Reaction Kit

Description

The Thermo Scientific™ Pierce Firefly Luciferase Glow Assay Kit provides a bright and stable bioluminescence signal (half-life greater than two hours) in the presence of firefly luciferase reporters for protein expression assays.

This Pierce Luciferase Glow Assay Kit contains reagents for measuring the activity of firefly luciferase in cell lysates. In the presence of firefly luciferase, the glow reagent provides stable bioluminescent signal and is highly suited for luminometers without injectors or for batch processing of samples. The light output generated by the luciferase reaction can be correlated with the amount of luciferase protein produced which in turn is proportional to the promoter activity driving the luciferase expression. The glow assay reagents were optimized for best results with Thermo Scientific Firefly Luc Plasmids; however, this assay kit may be used to detect the activity of other firefly or ATP-dependent luciferases that use D-luciferin as a substrate.

Highlights:

• Sensitive – highly sensitive detection of firefly luciferase activity; for best results (maximum signal) use with Thermo Scientific Pierce Firefly Luciferase Signal Enhancer

• Stable – increased signal stability compared to flash assays

• Simple – does not require a luminometer with injectors

• Compatible – assay reagents compatible with other firefly beetle luciferases

• Automation-friendly – amenable to high throughput assays

• Convenient – contains a universal cell lysis buffer and optimized glow assay reagent

• Safe – allows one to perform non-radioactive assays

Includes:

Cell lysis buffer, reaction buffer and substrate

Requires:

Firefly luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; For best results (maximum signal), use with Thermo Scientific Pierce Firefly Luciferase Signal Enhancer

Recommended for:

Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies

Specifications

• Type: Assay Kit
• Assay: Reporter Enzyme, Luciferase Reporter Assay
• Detection Method: Bioluminescence
• Format: 384-well plate, 96-well plate
• Substrate Properties: Chemical Substrate
• Substrate Type: Luciferase Substrate

• Target: Luciferase, Firefly Luciferase
• Compatible Cells: Mammalian Cells
• For Use With (Equipment): Luminometer (Microplate)
• Label Type: Enzyme Labeled
• Technique: Enhanced Chemiluminescence
• Substrate: D-Luciferin

For Research Use Only. Not for use in diagnostic procedures.