Thermo Scientific™ Fluoraldehyde™ o-Phthaldialdehyde Crystals (OPA)

Measure peptides or amino acids (Ex 340nm, Em 455nm) with phthalaldehyde, a fluorescent, amine-reactive, HPLC pre- or post-column labeling reagent.

Brand:  Thermo Scientific™ 26015

Product Code. 11821405

Description

Thermo Scientific™ Pierce Fluoraldehyde (OPA) is o-phthalaldehyde, a highly sensitive fluorescent pre- or post-column derivatization reagent for peptide or amino acid detection and quantitation in HPLC.

Fluoraldehyde Crystals are a specially purified, fluorogenic-grade preparation of o-phthalaldehyde (OPA). This amine-reactive compound is primarily used for high-sensitivity, fluorescent detection of primary amines, including amino acids, peptides, proteins and polyamines in pre- or post-column chromatographic effluents.

Highlights:

• For pre- or post-column amino acid derivatization for fluorescent detection and quantitation

• Reacts with all primary amine-containing analytes to yield fluorescent derivatives (ex/em = 340nm/455nm)

• Provides an accurate measure of both composition and absolute protein-peptide content

• Ideal for work with recombinant proteins and synthetic peptides

• Can be used for fluorescent protein or peptide assay

• Can be prepared as a stable aqueous solution in borate (see instructions)

Reagent Properties:

• Can be prepared as a stable aqueous solution in borate (see instructions)

• Alternative names: Fluoraldehyde Crystals, OPA

• Chemical name: ortho-Phthalaldehyde

• Reactive toward: Primary amines (–NH2)

• Chemical formula: C8H6O2

• CAS number: 643-79-8

• Molecular weight: 134.12

• Form: Pale yellow, free-flowing crystals

Specifications

• Product Type: o-Phthaldialdehyde
• Product Line: Fluoraldehyde™
• Label or Dye: OPA (o-phthaldialdehyde)
• Quantity: 5 g

• Chemical Reactivity: Amine
• Content And Storage: Store at room temperature.
• Detection Method: Fluorescence
• Form: Solid

Safety and Handling

Product Identifier

• o-Phthalaldehyde

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 3
• Serious eye damage/eye irritation Category 1
• Skin corrosion/irritation Category 1 B
• Skin sensitiser Category 1

Hazard Statement

• H301-Toxic if swallowed.
• H314-Causes severe skin burns and eye damage.
• H317-May cause an allergic skin reaction.
• H318-Causes serious eye damage.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash thoroughly after handling.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : phthalaldehyde

For Research Use Only. Not for use in diagnostic procedures.