Thermo Scientific™ Fluoraldehyde™ o-Phthaldialdehyde Reagent Solution (OPA)

Ready-to-use solution of o-phthalaldehyde, a fluorescent, amine-reactive, HPLC pre- or post-column labeling reagent to measure peptides or amino acids (Ex 340nm, Em 455nm).

Brand:  Thermo Scientific™ 26025

Product Code. 10530054

Description

Thermo Scientific™ Pierce Fluoraldehyde (OPA) Reagent is a ready-to-use solution of o-phthalaldehyde, a fluorescent pre- or post-column derivatization reagent for peptide or amino acid detection and quantitation in HPLC.

Fluoraldehyde Reagent contains OPA (o-phthalaldehyde), which reacts with primary amines of amino acids, peptide and proteins to enable fluorescent detection and quantitation. The reagent that can be used as a protein or peptide assay reagent or as a pre- or post-column detection reagent for amino acid analysis (HPLC). Reaction of OPA with proteins and peptides yields linear results over a wide range of concentrations. The Fluoraldehyde Reagent Solution is supplied ready to use and enables fast quantitation of proteins or peptides in solution.

Highlights:

• For pre- or post-column amino acid derivatization for fluorescent detection and quantitation

• Reacts with all primary amine-containing analytes to yield fluorescent isoindole derivatives

• Provides an accurate measure of both composition and absolute protein-peptide content

• Ideal for work with recombinant proteins and synthetic peptides

• Can be used for fluorescent protein or peptide assay

• Pre-column derivatization mixtures can be injected into LC without any processing

Reagent Properties:

• Formulation: 0.8mg/mL o-phthalaldehyde (OPA) in proprietary buffer, pH 10

• Form: Clear, colorless to slightly yellow liquid

• Reactive toward: Primary amines (–NH2)

• Detection: Fluorescence (excitation/emission = 340nm/455nm)

• Application: Pre- or post-column derivatization for detection by HPLC

Specifications

• Product Type: o-Phthaldialdehyde
• Product Line: Fluoraldehyde™
• Label or Dye: OPA (o-phthaldialdehyde)
• Quantity: 945 mL

• Chemical Reactivity: Amine
• Content And Storage: Store under nitrogen at 4°C away from oxidizing reagents and direct sunlight.
• Detection Method: Fluorescence
• Form: Liquid

Safety and Handling

Product Identifier

• Fluoraldehyde o-Phthaldialdehyde Reagent Solution (OPA)

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Skin sensitiser Category 1

Hazard Statement

• H315-Causes skin irritation.
• H317-May cause an allergic skin reaction.
• H319-Causes serious eye irritation.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : potassium hydroxide,2-mercaptoethanol

For Research Use Only. Not for use in diagnostic procedures.