Thermo Scientific™ Pierce™ Glycoprotein Staining Kit

Rapidly stain glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff (PAS) method in this convenient kit.

Brand:  Thermo Scientific™ 24562

Product Code. 10688864

Description

Thermo Scientific™ Pierce Glycoprotein Staining Kit is a convenient, fast and sensitive kit for staining glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff (PAS) method.

The kit provides three essential reagents and a complete protocol to specifically stain glycosylated proteins (glycoproteins) that have been separated by polyacrylamide gel electrophoresis (PAGE). A gel or membrane containing separated proteins is treated with a periodate solution, which oxidizes cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are detected through the formation of Schiff-base bonds with a reagent that produces magenta bands. Traditionally called the periodic acid-Schiff (PAS) reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions. The kit includes positive and negative control proteins.

Highlights:

• Specific for glycoproteins – periodic acid-Schiff (PAS) method specifically detects glycosylated proteins having sialic acid and other oxidizable carbohydrate groups

• Optimized protocol – the traditional three-reagent method has been streamlined to achieve optimal staining in less than two hours (half the time required for other staining methods)

• Colorimetric – glycoproteins are detected as magenta bands against a faint pink or colorless gel background

• Qualitative staining – detection limit depends on the extent of glycosylation and protein size and abundance; detects 0.625ng avidin and 0.16g horseradish peroxidase per band

• Controls included – complete kit includes positive and negative control proteins (HRP and STI, respectively).

• Gels and membranes – effective for use with polyacrylamide mini gels or nitrocellulose membranes

Includes:

Three reagents in 250mL bottles (1 solution and 2 dry powders to which water must be added), plus two vials of lyophilized proteins (1mg each)

Specifications

• Content And Storage: Sufficient For: 10 mini gels (SDS-PAGE)
  • Oxidation Reagent, 2.5 g powder to make 250 mL
  • Reduction Reagent, 1.25 g powder to make 250 mL
  • Positive Control (Horseradish Peroxidase), 1 mg
  • Negative Control (Soybean Trypsin Inhibitor), 1 mg
  • Glycoprotein Stain, 250 mL
  
  Store all components at 4°C.
• Detection Method: Colorimetric
• Product Type: Glycoprotein Staining Kit
• Quantity: 750 mL

• Detection Location: In-Blot Detection, In-Gel Detection
• Label or Dye: Fuchsin Sulfite
• Product Line: Pierce™
• Target Molecule: Proteins (Glycoproteins)

Safety and Handling

Product Identifier

• Pierce Glycoprotein Staining Kit

Signal Word

• Danger

Hazard Category

• Oxidising solid Category 2
• Acute toxicity Category 4
• Skin corrosion/irritation Category 1 B
• Serious eye damage/eye irritation Category 1
• Skin sensitiser Category 1
• Respiratory sensitiser Category 1
• Carcinogenicity Category 1B

Hazard Statement

• H272-May intensify fire; oxidiser.
• H302-Harmful if swallowed.
• H314-Causes severe skin burns and eye damage.
• H317-May cause an allergic skin reaction.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H350-May cause cancer.
• EUH031-Contact with acids liberates toxic gas.

Precautionary Statement

• P201-Obtain special instructions before use.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P284-¢In case of inadequate ventilation! wear respiratory protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P220-Keep/Store away from clothing/combustible materials.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P331-Do NOT induce vomiting.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305-IF IN EYES:.
• P310-Immediately call a POISON CENTER/doctor/.
• P501-Dispose of contents/container to .

For Research Use Only. Not for use in diagnostic procedures.