Thermo Scientific™ PageBlue™ Protein Staining Solution

A sensitive formulation of colloidal coomassie G-250 dye for endpoint staining of proteins in polyacrylamide gels and on PVDF membranes without methanol or acetic acid.

Brand:  Thermo Scientific™ 24620

Product Code. 11852174

Description

Thermo Scientific Pierce PageBlue Protein Staining Solution is a sensitive, economical formulation of colloidal coomassie G-250 dye for endpoint staining of proteins in polyacrylamide gels and on PVDF membranes without methanol or acetic acid.

The simple PageBlue Protein Staining Solution protocol is sensitive, efficient and eliminates the concern of over staining gels even after overnight staining. This protein stain delivers a dynamic range of 5 to 500ng, which is approximately 10 times more sensitive than traditional coomassie R-250-based dyes. Furthermore, PageBlue Protein Staining Solution is economical because it can be reused up to three times without any loss in sensitivity.

Highlights:

• Easy-to-use – simply soak the gel in the ready-to-use solution and observe stained bands without destaining

• Fast – 25 to 40 minute protocol

• Safe – does not contain methanol or acetic acid

• Dynamic range – linear dynamic range of 5 to 500ng

• Economical – can be reused up to 3 times

Recommended for:

Visualize and quantify proteins separated in 1D, 2D and IEF polyacrylamide gels; Stain proteins after transfer onto PVDF membranes; Stain gels prior to subsequent silver staining or mass spectrometry analysis

Specifications

• Content And Storage: Upon receipt store product at room temperature. Product shipped at ambient temperature
• Detection Method: Colorimetric
• Product Type: Protein Staining Solution
• Quantity: 1 L

• Detection Location: In-Blot Detection, In-Gel Detection
• Label or Dye: Coomassie
• Product Line: PageBlue™
• Target Molecule: Protein

Safety and Handling

Product Identifier

• PageBlue Protein Staining Solution, 1 L

Signal Word

• Danger

Hazard Category

• Flammable liquid Category 3
• Serious eye damage/eye irritation Category 1

Hazard Statement

• H226-Flammable liquid and vapour.
• H318-Causes serious eye damage.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P233-Keep container tightly closed.
• P243-Take precautionary measures against static discharge.
• P240-Ground/bond container and receiving equipment.
• P370+P378-In case of fire: Use to extinguish.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P310-Immediately call a POISON CENTER/doctor/.
• P403+P235-Store in a well-ventilated place. Keep cool.
• P501-Dispose of contents/container to .

Supplemental information

• MIXTURE LIST-Contains : Isopropyl alcohol, Phosphoric acid

For Research Use Only. Not for use in diagnostic procedures.