Thermo Scientific™ Pierce™ Peptide Retention Time Calibration Mixture

15 synthetic heavy peptides mixed in equimolar ratio to test and calibrate reverse-phase liquid chromatography (LC) and mass spectrometry (LC-MS) equipment.

Brand:  Thermo Scientific™ 88320

Product Code. 11572561

Description

Thermo Scientific™ Pierce Peptide Retention Time Calibration Mixture enables reversed phase liquid chromatography (LC) and mass spectrometry (LC-MS) users to optimize and confirm correct operation of their equipment.

The Pierce Peptide Retention Time Calibration Mixture is used to optimize and assess LC parameters, identify total peptide elution window and optimize MS parameters. Testing the mixture frequently throughout the lifetime of a column or including it in quantitative samples makes it possible to monitor and normalize LC-MS performance between samples and over time. When used with Thermo Scientific Pinpoint 1.1 software, the calibration mixture is used to predict peptide retention times based upon peptide sequence and ease the transfer of methods between LC-MS platforms.

Highlights:

• Assessment of chromatography and MS instrument performance
• Prediction of peptide retention across multiple instrument platforms
• Prediction of peptide retention time from sequence using calculated hydrophobicity factor
• Optimization of scheduled MS acquisition windows for improved quantification and increased multiplexing
• Internal standard to normalize for variation in retention times and peak intensities between runs

Specifications

• Description: Peptide Retention Time Calibration Mixture
• Product Type: Peptide Retention Time Calibration Mixture
• Quantity: 50 μL

• Formulation: Mixture of 15 synthetic heavy peptides at 0.5uM each
• Sufficient For: 5 to 100 uses
• For Use With (Application): LC-MS

Safety and Handling

Product Identifier

• Pierce Peptide Retention Time Calibration Mixture

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : acetonitrile