Thermo Scientific™ EZ-Link™ Plus Activated Peroxidase Kit

Periodate-aldehyde-activated horseradish peroxidase (HRP) enzyme and kit to label primary antibodies to obtain high-activity antibody-HRP conjugates.

Brand:  Thermo Scientific™ 31489

Product Code. 10036673

Description

Thermo Scientific™ EZ-Link™ Plus Activated Peroxidase Kit contains horseradish peroxidase enzyme that has been aldehyde-activated with periodate to enable high-efficiency labeling and preparation of protein or antibody conjugates.

Other amine-reactive chemistries, like glutaraldehyde, have a tendency to polymerize and produce lower amounts of viable conjugate. Sugar residues present on HRP have been oxidized with periodate to produce aldehydes that react with primary amines. Plus Activated Peroxidase is mixed with the protein to be coupled at a pH compatible with your protein or antibody. After incubating the reaction for one to two hours, the conjugate linkage is reduced and the activated HRP quenched with ethanolamine. The conjugate is purified by desalting or by dialysis. If you are preparing an antibody-HRP conjugate, use the Thermo Scientific Pierce Conjugate Purification Kit (Part No. 44920) to separate unreacted enzyme from the conjugate.

Highlights:

• Reacts with readily accessible primary amines to form a covalent secondary amine bond
• Can be stored for at least 12 months at -20°C
• Consistent conjugation yields reliable conjugates
• Enzyme activity is 120-200 units/mg

Specifications

• Quantity: 1-plate kit
• Product Line: EZ-Link™
• Labeling Target: Antibodies (General), Proteins (General)
• Labeling Scale: 0.5-1 mg

• Content And Storage: Sufficient For: 5 enzyme-labeling reactions, each with up to 1 mg of antibody (IgG)
  • Plus Activated Peroxidase, 5 x 1 mg
  • Sodium cyanoborohydride solution, 1 x 0.5 mL
  • Quenching Buffer, 25 mL
  • PBS Pack (makes 500 mL), 1 pack
  • Carbonate-Bicarbonate Buffer Pack (makes 500 mL), 1 pack
  
  Store at -20°C.
• Sufficient For: 5 Enzyme Labeling Reactions
• Label or Dye: HRP (Horseradish Peroxidase)

Safety and Handling

Product Identifier

• Z-Link Plus Activated Peroxidase Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 2
• ACUTE AQUATIC Acute 1
• Serious eye damage/eye irritation Category 1
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 1 B
• Specific target organ toxicity Category 3

Hazard Statement

• H300-Fatal if swallowed.
• H310-Fatal in contact with skin.
• H314-Causes severe skin burns and eye damage.
• H318-Causes serious eye damage.
• H319-Causes serious eye irritation.
• H330-Fatal if inhaled.
• H335-May cause respiratory irritation.
• H400-Very toxic to aquatic life.
• EUH032-Contact with acids liberates very toxic gas.
• EUH204-Contains isocyanates. May produce an allergic reaction.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P262-Do not get in eyes, on skin, or on clothing.
• P264-Wash hands thoroughly after handling.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P331-Do NOT induce vomiting.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Sodium carbonate, Sodium hydroxide, Ethanolamine

For Research Use Only. Not for use in diagnostic procedures.