Thermo Scientific™ Pierce™ Power Blotter

Get rapid and efficient protein transfer at your fingertips with the Thermo Scientific™ Pierce™ Power Blotter.

Product Code	Brand	Description	Includes	For Use With (Application)
15635315	Thermo Scientific™ 22834	Pierce Power Blotter	Pierce Power Station, Pierce Power Blot Cassette, Western Blot Roller, Power Cord with C/13 Connector, Quick Start Guide	Semi-dry transfer of proteins from polyacrylamide gels to membranes
15319134	Thermo Scientific™ 22834SPCL	Pierce™ Power Blotter Welcome Pack, SDS-PAGE Gels Compatibility, Horizontal Running Dimension, Semi-Dry Mode of Transfer, 100 to 240V, 50/60Hz, 168W Electrical Requirements, Use With iBlot™, For Protein Assays and Analysis	Pierce power station, pierce power blot cassette, western blot roller, power cord with C/13 connector, quick start guide, pierce 1-Step transfer buffer, 1L western blotting filter paper, 7 x 8.4cm, 100 sheets	Protein assays and analysis, protein gel electrophoresis
15399114	Thermo Scientific™ 22835	Pierce Power Blot Cassette	Pierce Power Blot Cassette	Semi-dry transfer when used with Pierce Power Station

Description

Get rapid and efficient protein transfer at your fingertips with the Thermo Scientific™ Pierce™ Power Blotter.

The Thermo Scientific Pierce Power Blotter is designed specifically for rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels to nitrocellulose or PVDF membranes in 5 to 10 minutes.

The Pierce Power Blotter replaces the Pierce G2 Fast Blotter and features an integrated power supply optimized to deliver consistent, high-efficiency protein transfer when used with commonly used precast or homemade gels (SDS-PAGE) and nitrocellulose or PVDF membranes. The Power Blot cassette enables the simultaneous transfer of up to four mini-sized gels or two midi-sized gels. The Power Station component of the Power Blotter has an easy-to-use color LCD touchscreen interface and pre-programmed transfer methods for different numbers and sizes of gels and ranges of protein molecular weight. The easy-touch programming feature allows custom transfer settings to be quickly created, saved and run.

The Pierce Power Blotter achieves highly efficient transfer in less than 10 minutes when used with Pierce 1-Step Transfer Buffer. The instrument is also effective for standard 30- to 60-minute semi-dry transfer protocols based on traditional buffers.

Highlights:

• Efficient – achieve high transfer efficiency with a broad range of protein sizes (10-300kDa) compared to conventional semi-dry or wet (tank) transfer methods
• Fast – transfer proteins in 5 to 10 minutes when used with 1-Step Transfer Buffer
• Integrated power supply – seamless operation between control unit and cassette provides consistent high efficiency protein transfer
• Easy-touch programming – access pre-programmed transfer methods or create, save and run customized transfer methods
• Convenient – simultaneously transfer 1 to 4 mini gels or 1 to 2 medium-sized gels
• Versatile – use with Pierce 1-Step Transfer Buffer for rapid blotting programs or Towbin transfer buffer for conventional semi-dry transfer methods
• Upgrade-ready – adding the Pierce Power Stain Cassette activates the pre-loaded software making this unit a fully functional Pierce Power System with gel staining and blotting capabilities.

Includes:

• System consists of the Pierce Power Station and Pierce Power Blot Cassette; kit includes Western Blot Roller, Power Cord with C/13 Connector, and Quick Start Guide
• Unit ships with North American power cord; country-specific power cords are also available

Applications:

• Semi-dry Western blot transfer of 10-300kDa proteins from polyacrylamide gels to nitrocellulose or PVDF membranes in about 10 minutes using optimized transfer buffer

Optional:

• Pierce Power Stain Cassette (22836) - activates pre-loaded software to enable electrophoretic protein staining

Product Details:

Traditional wet blotting techniques require transfer times of one hour to overnight to achieve good transfer efficiency. When used in conjunction with Pierce 1-Step Transfer Buffer, the Pierce Power Blotter provides efficient protein transfer in 5-10 minutes with results equivalent to, or better than, traditional Western blot transfer techniques. This system also eliminates the need for gel pre-equilibration. This significant reduction in protein transfer time is accomplished by optimizing the ionic strength of the transfer buffer and increasing the current [amps (A)/cm2] flowing through the transfer stack. The Pierce Power Blotter can also be used for standard 30- to 60-minute semi-dry transfer protocols with Towbin buffer (Towbin et al., 1979).