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Thermo Scientific™ Pierce™ Premium Grade Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)
Optimize applications where product integrity and risk minimization are paramount our highest quality formulation of this popular amine-to-sulfhydryl crosslinker.
Brand: Thermo Scientific™ PG82085
Description
Optimize applications where product integrity and risk minimization are paramount with the specially characterized Thermo Scientific™ Pierce™ Premium Grade Sulfo-SMCC, our highest quality formulation of this popular amine-to-sulfhydryl crosslinker.
Thermo Scientific Pierce Premium Grade Sulfo-SMCC is our highest quality formulation of this popular amine-to-sulfhydryl crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.
Compared to the standard grade product, Premium Grade Sulfo-SMCC provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. Sulfo-SMCC is a water-soluble, non-cleavable and membrane-impermeable amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm (8.3 angstroms). NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of Sulfo-SMCC are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is soluble up to nearly 10mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure.
Highlights:- Sulfo-SMCC – a water-soluble amine-to-sulfhydryl crosslinker with a stabilizing cyclohexane spacer arm
- High quality – identity and purity confirmed by several tests, including quantitative NMR
- Product integrity – enhanced level of testing and characterization compared to standard grade
- Lot retention – ample supply of past lots retained to ensure future process testing
- Change management – Change Control Notification (CCN) service
- Consistent manufacture – batch-specific manufacturing documentation review
- Enzyme labeling of antibodies - both enzyme and antibody specificity can be preserved
- Create specific bioconjugates via one- or two-step crosslinking reactions
- Create sulfhydryl-reactive, maleimide-activated carrier proteins for coupling haptens
Specifications
Amine-Sulfhydryl | |
436.37 | |
8.3 Å | |
No | |
Pierce™ | |
Heterobifunctional | |
Short (<10 Å) | |
100 mg | |
Standard, Single-use, Premium-grade | |
Crosslinker |
No | |
No | |
Upon receipt store desiccated at -20°C. | |
Ambient | |
Chemical Labeling | |
Maleimide, Sulfo-NHS Ester | |
Powder | |
Water | |
Yes |
Safety and Handling
- Pierce Premium Grade Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
Precautionary Statement
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Cyclohexanecarboxylic acid, 4-[(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)methyl]-, 2,5-dioxo-3-sulfo-1-p
For Research Use Only. Not for use in diagnostic procedures.