Thermo Scientific™ Pierce™ Premium Grade Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)

Optimize applications where product integrity and risk minimization are paramount our highest quality formulation of this popular amine-to-sulfhydryl crosslinker.

Product Code	Brand	Description
15205773	Thermo Scientific™ PG82085	Sulfo-SMCC
15215773	Thermo Scientific™ PG82086	Sulfo-SMCC

Description

Optimize applications where product integrity and risk minimization are paramount with the specially characterized Thermo Scientific™ Pierce™ Premium Grade Sulfo-SMCC, our highest quality formulation of this popular amine-to-sulfhydryl crosslinker.

Thermo Scientific Pierce Premium Grade Sulfo-SMCC is our highest quality formulation of this popular amine-to-sulfhydryl crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.

Compared to the standard grade product, Premium Grade Sulfo-SMCC provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. Sulfo-SMCC is a water-soluble, non-cleavable and membrane-impermeable amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm (8.3 angstroms). NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of Sulfo-SMCC are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is soluble up to nearly 10mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure.

Highlights:

• Sulfo-SMCC – a water-soluble amine-to-sulfhydryl crosslinker with a stabilizing cyclohexane spacer arm
• High quality – identity and purity confirmed by several tests, including quantitative NMR
• Product integrity – enhanced level of testing and characterization compared to standard grade
• Lot retention – ample supply of past lots retained to ensure future process testing
• Change management – Change Control Notification (CCN) service
• Consistent manufacture – batch-specific manufacturing documentation review

Applications:

• Enzyme labeling of antibodies - both enzyme and antibody specificity can be preserved
• Create specific bioconjugates via one- or two-step crosslinking reactions
• Create sulfhydryl-reactive, maleimide-activated carrier proteins for coupling haptens

Specifications

• Chemical Reactivity: Amine-Sulfhydryl
• Description: Sulfo-SMCC
• PEGylated: No
• Content And Storage: Upon receipt store desiccated at -20°C.
• Shipping Condition: Ambient
• Labeling Method: Chemical Labeling
• Reactive Moiety: Maleimide, Sulfo-NHS Ester
• Form: Powder
• Format: Standard, Single-use, Premium-grade
• Product Type: Crosslinker

• Cleavable: No
• Molecular Weight (g/mol): 436.37
• Spacer Arm Length: 8.3 Å
• Cell Permeability: No
• Product Line: Pierce™
• Crosslinker Type: Heterobifunctional
• Spacer: Short (<10 Å)
• Solubility: Water
• Water Soluble: Yes

Safety and Handling

Product Identifier

• Pierce Premium Grade Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Cyclohexanecarboxylic acid, 4-[(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)methyl]-, 2,5-dioxo-3-sulfo-1-p

For Research Use Only. Not for use in diagnostic procedures.