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Thermo Scientific™ Pierce™ Fluorescent Protease Assay Kit
Determine total protease activity in biological samples via fluorescent or colorimetric detection by measuring digestion of labeled casein substrate.
Brand: Thermo Scientific™ 23266
261.86 GBP valid until 2024-11-29
Use promo code "21683" to get your promotional price.
Description
Thermo Scientific™ Pierce Protease Assay Kits measure total protease activity in samples, providing a means of assessing the progress of protease isolation procedures or quantifying protease contamination in biological samples.
These Colorimetric and Fluorescent Protease Assay Kits comprise two different methods for measuring total protease activity in biological samples. Both kits can be used to assess the progress of protease purification procedures or to quantify protease contamination in protein samples. The kits use succinylated or fluorescein-tagged casein as a protease substrate. Proteolysis of the substrate results in peptides whose amino groups or altered fluorescence properties enable detection in the respective kits. Protease activity is quantified by comparison to trypsin standard included in each kit.Highlights:
Casein substrate – measure activity of any protease that cleaves casein into peptide fragments
Trypsin standard – quantify protease activity relative to trypsin, a universally accepted reference
Sensitive – 1000 times more sensitive than assays that use unmodified forms of casein
Simple and fast – test tube and microplate protocols are completed in less than one hour
Homogenous – addition steps only; no separation, transfer or stop steps required
Customizable – easily adapt time, temperature and pH to optimize sensitivity for proteases of interest
- Substrate: succinylated casein (not available separately)
- Basis of assay: detection of primary amines resulting from proteolysis
- Detection reagent: trinitrobenzene sulfonic acid (TNBSA)
- Measurement: absorbance at 450nm (plate reader or spectrophotometer)
- Substrate: fluorescein-labeled casein (FITC-casein); available separately as Part No. 23267
- Basis of assay: change in fluorescence resonance (FRET) or fluorescence polarization (FP)
- Measurement: instrument with fluorescein excitation and emission filters (485/538nm)
Includes:
Modified casein, trypsin standard and Tris-buffered saline (both kits); TNBSA solution (Part No. 23263 only)
Recommended for:
Assess functional integrity and overall progress of protease purification; Quantify protease contamination in protein samples; Characterize the activity of unusual proteases compared to trypsin; Evaluate buffer conditions for their affects on protease function and activity
Specifications
Sufficient For: 2000 microplate assays using an instrument fitted with a fluorescein filter set (ex/em 485 nm/538 nm) • FTC-Casein, lyophilized, 2.5 mg • TPCK Trypsin Standard, 50 mg • TBS Buffer Pack (makes 500 mL), 1 pack Store FTC-Casein and TPCK Trypsin at 4°C. |
|
Fluorescence | |
Classic Dye | |
Protein Activity Assay | |
Protein-Based Substrate | |
2000 Microplate Assays | |
Microplate Reader | |
Proteases |
Protease Assay | |
96-well Plate | |
Pierce™ | |
Casein | |
Protease Substrate | |
Fluorescence Polarization, FRET | |
2000 Test Kit |
Safety and Handling
- Pierce Fluorescent Protease Assay Kit
Signal Word
- Danger
Hazard Category
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
- Skin corrosion/irritation Category 2
- Skin sensitiser Category 1
- Specific target organ toxicity Category 3
Hazard Statement
- H315-Causes skin irritation.
- H317-May cause an allergic skin reaction.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H335-May cause respiratory irritation.
Precautionary Statement
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P272-Contaminated work clothing should not be allowed out of the workplace.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P284-¢In case of inadequate ventilation! wear respiratory protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
- P363-Wash contaminated clothing before reuse.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Sodium chloride, Tris (hydroxymethyl) aminomethane, Fluoresceinthiocarbamoyl-casein, Trypsin
For Research Use Only. Not for use in diagnostic procedures.