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Thermo Scientific™ Pierce™ Fluorescent Protease Assay Kit

Determine total protease activity in biological samples via fluorescent or colorimetric detection by measuring digestion of labeled casein substrate.

Brand:  Thermo Scientific™ 23266

261.86 GBP valid until 2024-11-29
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Product Code. 10485495

  • £278.00 / 1 set
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Description

Description

Thermo Scientific™ Pierce Protease Assay Kits measure total protease activity in samples, providing a means of assessing the progress of protease isolation procedures or quantifying protease contamination in biological samples.

These Colorimetric and Fluorescent Protease Assay Kits comprise two different methods for measuring total protease activity in biological samples. Both kits can be used to assess the progress of protease purification procedures or to quantify protease contamination in protein samples. The kits use succinylated or fluorescein-tagged casein as a protease substrate. Proteolysis of the substrate results in peptides whose amino groups or altered fluorescence properties enable detection in the respective kits. Protease activity is quantified by comparison to trypsin standard included in each kit.

Highlights:

  • Casein substrate – measure activity of any protease that cleaves casein into peptide fragments

  • Trypsin standard – quantify protease activity relative to trypsin, a universally accepted reference

  • Sensitive – 1000 times more sensitive than assays that use unmodified forms of casein

  • Simple and fast – test tube and microplate protocols are completed in less than one hour

  • Homogenous – addition steps only; no separation, transfer or stop steps required

  • Customizable – easily adapt time, temperature and pH to optimize sensitivity for proteases of interest

The Colorimetric Protease Assay Kit (Part No. 23263) uses fully succinylated casein as a substrate for the assay. Hydrolysis of this substrate in the presence of protease results in the release of peptide fragments with free amino-terminal groups. These peptides are reacted with trinitrobenzene sulfonic acid (TNBSA), followed by measurement of the absorbance increase that results from the formation of yellow colored TNB-peptide adducts.
  • Substrate: succinylated casein (not available separately)
  • Basis of assay: detection of primary amines resulting from proteolysis
  • Detection reagent: trinitrobenzene sulfonic acid (TNBSA)
  • Measurement: absorbance at 450nm (plate reader or spectrophotometer)
The Fluorescent Protease Assay Kit (Part No. 23266) includes fluorescein-labeled casein as a substrate for assessing protease activity in a sample by either fluorescence resonance energy transfer (FRET) with a standard fluorometer or fluorescence polarization (FP) with capable instrumentation. FTC-Casein is native casein that has been labeled using a large molar excess of fluorescein isothiocyanate (FITC). Fluorescence properties of this heavily-labeled, intact protein substrate change dramatically upon digestion by proteases, resulting in a measurable indication of proteolysis.
  • Substrate: fluorescein-labeled casein (FITC-casein); available separately as Part No. 23267
  • Basis of assay: change in fluorescence resonance (FRET) or fluorescence polarization (FP)
  • Measurement: instrument with fluorescein excitation and emission filters (485/538nm)

Includes:

Modified casein, trypsin standard and Tris-buffered saline (both kits); TNBSA solution (Part No. 23263 only)

Recommended for:

Assess functional integrity and overall progress of protease purification; Quantify protease contamination in protein samples; Characterize the activity of unusual proteases compared to trypsin; Evaluate buffer conditions for their affects on protease function and activity

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Sufficient For: 2000 microplate assays using an instrument fitted with a fluorescein filter set (ex/em 485 nm/538 nm)
• FTC-Casein, lyophilized, 2.5 mg
• TPCK Trypsin Standard, 50 mg
• TBS Buffer Pack (makes 500 mL), 1 pack

Store FTC-Casein and TPCK Trypsin at 4°C.
Fluorescence
Classic Dye
Protein Activity Assay
Protein-Based Substrate
2000 Microplate Assays
Microplate Reader
Proteases
Protease Assay
96-well Plate
Pierce™
Casein
Protease Substrate
Fluorescence Polarization, FRET
2000 Test Kit
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Safety and Handling

Safety and Handling

Product Identifier
  • Pierce Fluorescent Protease Assay Kit

Signal Word
  • Danger

Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 2
  • Skin sensitiser Category 1
  • Specific target organ toxicity Category 3

Hazard Statement
  • H315-Causes skin irritation.
  • H317-May cause an allergic skin reaction.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H335-May cause respiratory irritation.

Precautionary Statement
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P272-Contaminated work clothing should not be allowed out of the workplace.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P284-¢In case of inadequate ventilation! wear respiratory protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
  • P363-Wash contaminated clothing before reuse.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : Sodium chloride, Tris (hydroxymethyl) aminomethane, Fluoresceinthiocarbamoyl-casein, Trypsin

SDS
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For Research Use Only. Not for use in diagnostic procedures.