Invitrogen™ Platinum™ Green Hot Start PCR Master Mix (2X)

Invitrogen Platinum Green Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup.

Brand:  Invitrogen™ 13001013

Product Code. 15420594

Description

Invitrogen Platinum Green Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup. It retains all the features of Platinum Taq DNA Polymerase: specificity, robustness, and reliability. Platinum Green Hot Start PCR Master Mix is supplemented with two tracking dyes for the convenience of direct gel loading of PCR products.

Features of Platinum Green Hot Start PCR Master Mix include:

• High specificity and increased yields with antibody-mediated hot-start PCR
• Convenient room-temperature reaction setup
• Direct gel loading with green PCR master mix
• Versatile formulation for broad range of amplicons

About Platinum Taq DNA Polymerase

Platinum Taq DNA Polymerase is a recombinant Taq DNA polymerase complexed with proprietary antibodies that inhibit enzyme activity at room temperature, reducing nonspecific target amplification at lower temperatures. The antibodies dissociate during the initial PCR denaturation step and DNA polymerase regains its full activity. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5'→3' exonuclease activity.

Using Platinum Green Hot Start PCR Master Mix

Convenient Platinum Green Hot Start PCR Master Mix provides a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup. The two tracking dyes in Platinum Green Hot Start PCR Master Mix enable direct gel loading of PCR products, thus eliminating tedious steps of dye addition. Platinum Green Hot Start PCR Master Mix is supplied with a separate vial of Platinum GC Enhancer formulated for specific amplification and improved yields of GC-rich targets. Use Platinum Green Hot Start PCR Master Mix in PCR applications such as genotyping, gene expression profiling, and next-generation sequencing.

Note: For superior PCR performance, we recommend next generation enzyme Platinum II Taq Hot-start DNA Polymerase. Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology. Platinum II Hot-Start Green PCR Master Mix contains Platinum II Taq Hot-Start DNA Polymerase in a ready-to-use mixture with Platinum II PCR buffer and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup. It additionally contains a density reagent and two tracking dyes for direct loading of PCR products on gels.

Specifications

• Content And Storage: • Platinum Green Hot Start PCR 2X Master Mix, 4 x 1.25 mL
  • Platinum GC Enhancer, 2 x 1.25 mL
  • Nuclease-free water, 4 x 1.25 mL
  
  Store at -20°C in a non-frost-free freezer.
• GC-Rich PCR Performance: Medium
• Reaction Speed: Standard
• Product Type: Hot Start PCR Master Mix
• For Use With (Application): Hot-start PCR
• Fidelity (vs. Taq): 1X
• No. of Reactions: 200 Reactions
• Reaction Format: SuperMix or Master Mix
• Starting Material: DNA

• Format: Tube
• Polymerase: Platinum Taq DNA Polymerase
• Label or Dye: Green
• Shipping Condition: Dry Ice
• Concentration: 2X
• Hot Start: Built-In Hot Start
• Overhang: 3'-A
• Size (Final Product): 5 kb or less

For Research Use Only. Not for use in diagnostic procedures.