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Invitrogen™ Platinum™ Taq DNA Polymerase, DNA-free
Invitrogen Platinum Taq DNA Polymerase, DNA-free, is manufactured using a novel, closed single-use system together with stringent quality-control testing to minimize the risk of contamination with bacterial and human DNA.
Brand: Invitrogen™ 15966005
230.25 GBP valid until 2024-12-31
Use promo code "21651" to get your promotional price.
Description
Invitrogen Platinum Taq DNA Polymerase, DNA-free, is manufactured using a novel, closed single-use system together with stringent quality-control testing to minimize the risk of contamination with bacterial and human DNA. This enzyme is an ideal choice for PCR-based applications requiring the high sensitivity without false-positive results from reagent-borne contamination. This DNA-free enzyme provides the same high level of performance and lot-to-lot consistency as standard Platinum Taq DNA Polymerase.
Platinum Taq DNA Polymerase, DNA-free, is a recombinant Taq polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures and dissociates after the initial denaturation step at 94°C. This automatic hot start provides increased sensitivity, specificity, and yield, while allowing convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase, DNA-free, has a non-template–dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity.
Features
- Certified low DNA contamination level (≤0.01 copy of bacterial gDNA per enzyme unit)
- Confidence in qualitative and quantitative detection of low-abundance microorganisms due to signal absence with no-template/reagents-only PCR controls
- Same performance as Platinum Taq DNA Polymerase manufactured using conventional process
Applications
- Amplification of DNA from complex genomic and viral templates
- Hot start PCR
- RT-PCR
Specifications
• Platinum Taq DNA Polymerase, DNA-free, 100 μL • 10X PCR Buffer (without magnesium), DNA-free, 1.25 mL • 50 mM MgCl2, DNA-free (1 mL) Store at -20°C in a non-frost-free freezer. |
|
Low | |
Standard | |
500 Units | |
Hot-start PCR | |
1X | |
400 Reactions | |
Separate Components | |
DNA |
Tube | |
Platinum Taq DNA Polymerase | |
Taq DNA Polymerase | |
Dry Ice | |
5 U/μL | |
Built-In Hot Start | |
3'-A | |
5 kb or less |
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For Research Use Only. Not for use in diagnostic procedures.