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Thermo Scientific™ PNPP Substrates
Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.
£160.00 - £240.00
Specifications
| Product Type | ELISA Substrate |
|---|---|
| Detection Method | Colorimetric |
| Substrate Properties | Chemical Substrate |
| Substrate Type | Alkaline Phosphatase |
| Product Code | Brand | Description | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Description | Price | Quantity & Availability | |||||
|
11889260
|
Thermo Scientific™
34045 |
PNPP Powder |
£185.00
25g |
Estimated Shipment: 07-01-2025
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|
11859270
|
Thermo Scientific™
34047 |
PNPP Tablets |
£191.00
105 tablets |
In Stock
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|
10571384
|
Thermo Scientific™
37620 |
PNPP Substrate Kit |
£240.00
1 set |
In Stock
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|
10371394
|
Thermo Scientific™
37621 |
1-Step™ PNPP Substrate Solution |
£160.00
100mL |
In Stock
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Description
PNPP (p-Nitrophenyl Phosphate, Disodium Sal) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution, and a complete kit containing substrate solution and diethanolamine buffer.
Thermo Scientific Pierce PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm.
Features of PNPP (p-nitrophenyl phosphate):
• One-component alkaline phosphatase (AP) substrate
• Absorbance maximum of 405 nm
• Develops a yellow hue in solution upon reaction with AP
• Safer to use—contains a non-mercury, non-azide preservative
The substrate is offered in several formats:
PNPP Substrate
• Powder Form
PNPP Substrate Tablets
• 5 mg tablets with an inert filler as the balance
PNPP Substrate Kit
• PNPP tablets and a Diethanolamine Buffer to yield more than one liter of substrate. The Diethanolamine Substrate Buffer (Cat. No. 34064) is also provided individually as a 5X concentrate.
1-Step™ PNPP Substrate Solution
• Circumvents this time-consuming and variable introducing step by providing a single component PNPP substrate. This substrate is stable for 12 months at 2–8°C and can be stopped with conventional methods.
Specifications
| ELISA Substrate | |
| Colorimetric | |
| Chemical Substrate | |
| Alkaline Phosphatase |
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