Pre-Sort Buffer (RUO), BD Biosciences™

Designed to aid in preparing and maintaining single cell suspensions prepared from lymphoid tissues, bone marrow, peripheral blood, cultured cells (including pluripotent stem cells) or other sources.

Brand:  BD Biosciences 563503

Product Code. 15842839

Description

• Designed to aid in preparing and maintaining single cell suspensions prepared from lymphoid tissues, bone marrow, peripheral blood, cultured cells (including pluripotent stem cells) or other sources.
• Additives may be necessary for sensitive cell types requiring specific nutrients.
• The Pre-Sort Buffer contains no phenol red and is an optically clear buffer to help minimize background.
• The buffer has been formulated to minimize cell clumping which facilitates consistent drop formation on fluorescence activated cell sorters.
• The buffer has minimal calcium and magnesium to minimize cell aggregation as these cations are necessary cofactors for many cell adhesion molecules.
• The Pre-Sort Buffer contains an FBS-based protein to help maintain cells in a viable state during cell sorting applications but the buffer does not contain EDTA, which can have deleterious effects on cells.
• It is useful for the dilution and application of fluorescent reagents as well as for the suspension, washing, and storage of cells destined for florescence activated cell sorting (FACS) or flow cytometric analysis.

Specifications

• Quantity: 250 mL
• Formulation: Frozen aqueous buffered solution containing no preservative, 0.2 μm sterile filtered. Endotoxin level is ≤0.96 EU/mL as determined by the LAL assay.