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Invitrogen™ Prostasin/PRSS8 Human ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ EHPRSS8X5
Description
The Human Prostasin/PRSS8 ELISA quantitates Hu Prostasin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Prostasin. Principle of the method The Human Prostasin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.Specifications
Q16651 | |
30 pg/mL | |
Solid-phase sandwich ELISA | |
ELISA Kit | |
Plasma, Serum, Supernatant | |
5 x 96 assays | |
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
5652 | |
<12% | |
Wash Buffer, Assay Diluent Concentrate, Standard, Stop Solution, Biotinylated Detection Antibody, Pre-coated 96 well plates, Adhesive Plate Covers, Chromogen, SAV-HRP | |
5 x 96 Tests | |
Cell Cycle & Proliferation | |
2°C to 8°C | |
Human | |
4 hr. 45 min. |
32.77 pg/mL to 8000 pg/mL | |
30 pg/mL to 8000 pg/mL | |
Biotin | |
Cell Structure & Organelle Proteins | |
This ELISA kit shows no cross-reactivity with the following cytokines tested: human ANGPTL3, beta IG-H3, CA9, Cathepsin B, CD23, CHI3L1, CTLA-4, DKK-4, DPPIV, EDA-A2, Erythropoietin R, FGF-6, FGF-9, Gas 1, GITR, IGFBP-5, IL-1 F5, IL-1 F6, IL-1 F7, IL-1 F8, IL-1 F9, IL-1 F10, IL-1 R5, IL-21, IL-18, IL-20, IL-34, IL-5 R alpha, IL-10 R alpha, Layilin, Leptin R, Marapsin, Mer, MMP-7, P-Cadherin, PSMA, SIGIRR, TGF beta R3, Coagulation Factor III, TWEAK. | |
Human | |
Colorimetric Microplate Reader | |
PRSS8 | |
<10% | |
HRP | |
RUO | |
Plasma, 50 μ;L; Serum, 50 μ;L; Supernatant, 100 μ;L | |
CAP1, PROSTASIN | |
1 hr. 20 min. |