Macherey-Nagel™ Protein Quantification Assay

NucleoSpin™ miRNA is suitable for the parallel isolation of small RNA, large RNA and protein

Product Code	Brand	Assay
12779650	Macherey-Nagel™ 740967.250	250
11922362	Macherey-Nagel™ 740967.50	50

Description

NucleoSpin™ miRNA is suitable for the parallel isolation of small RNA, large RNA and protein.
Superior selectivity - purify your RNA fractionated by size
Isolation of small RNA only (18 to 200 bases)Isolation of small (18 to 200 bases) and large (>200 bases) RNA in two fractionsIsolation of total RNA (small and large in one fraction)
Additional isolation of total protein
Denatured protein ready-to-use for analysis in SDS-PAGE and Western blot.
Excellent RNA recovery - even without phenol/chloroform
Lysis with chaotropic saltSpin column based procedure
Convenient handling - complete equipment provided
NucleoSpin filters - efficient sample homogenisationNucleoSpin protein removal columns - highest purity of small RNArDNase - highly efficient on-column digestion of genomic DNA at RT

• Silica membrane technology
• Format: minispin columns
• Sample size: <10⁷ cultured cells, <30mg human/tissue; <50mg plant tissue, <150μL reaction mixture
• Fragment size, small RNA: 18 to 200 bases
• Fragment size, large RNA: >200 bases
• Typical yield: 10μg small RNA; 95μg large RNA from 10⁷ HeLa cells
• Binding capacity: 200μg
• Elution volume, 30μL to 100μL
• Preparation time: <45min (6 preps human/animal tissue, small and large RNA); <35min (6 preps human/animal tissue, small RNA only)
• Small RNA (18 to 200 bases)
• Large RNA (>200 bases)
• Total RNA (small and large RNA in one fraction), and isolate your total protein fraction For small and large RNA and total RNA purification, and isolation of total protein fraction.

Specifications

• Concentration: 10 to 20000ng/μL
• Immunoassay Kit Format: Buffer and Reagent Set
• Sample Type: Protein
• For Use With (Application): Small and large RNA and total RNA purification
• Quantity: 1 Each
• Total Assay Time: 40 min.

• Detectable Analytes: Protein
• Product Type: Protein Quantification Assay
• Sufficient For: 107
• Kit Contents: Chaotropic salt
• Test Time: 40 min.

Safety and Handling

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Substances and mixtures corrosive to metals Category 1
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H290-May be corrosive to metals.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.

Supplemental information

• MIXTURE LIST-Contains : hydrochloric acid