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Invitrogen™ PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit

The PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange membrane.

Brand:  Invitrogen™ A31217


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Product Code. 15722362

  • £312.00 / Pack of 10

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Description

Description

The PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange membrane. In as little as 90 minutes, purify up to 1.5 mg of high-quality plasmid DNA that is suitable for advanced transfection applications such as transfection of sensitive cells lines or genome engineering.

Advantages of the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit include:
• Simple and fast protocol—purification typically takes 90 minutes
• High yield—isolate up to 1.5 mg of high quality plasmid DNA
• Endotoxin-free plasmid—less than 0.1 EU/μg, ideal for sensitive applications
• High purity—ultralow levels of protein, RNA, and genomic DNA contamination
• Compatibility—suitable for use with ExpiCHO and Expi293 expression systems
• Endotoxin-free components— elution buffer, water, and plasmid storage tubes that are free of endotoxin

Simple and fast protocol
Unlike other anion-exchange-based endotoxin-free plasmid purification systems, the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit utilizes centrifugation- or vacuum-assisted filtration for lysate clarification and subsequent plasmid DNA purification. There is no waiting for gravity-flow columns and the entire purification typically takes 90 minutes (including the final precipitation step).

Endotoxin-free plasmid
Purify endotoxin-free (less than 0.1 EU/μg) plasmid that is ideal for sensitive applications such as transfection of primary cells, genome engineering, and research on gene therapy or plasmid vaccines in vivo.

Compatible with ExpiCHO and Expi293 expression systems
Plasmid purified using the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit is fully compatible with the Gibco ExpiCHO and Expi293 transient mammalian expression systems.

Superior quality with endotoxin-free components
Rest assured that the kit is of high quality and has additional endotoxin-free elution buffer, water, and plasmid storage tubes for added peace of mind.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

• Resuspension buffer
• Lysis indicator
• RNase A
• Lysis buffer
• Precipitation buffer
• Endotoxin removal buffer
• Wash buffer
• Elution buffer
• TE buffer
• Expi Maxi filtration column
• Expi Maxi DNA binding column
• 50 mL conical tubes

Endotoxin-free components: elution buffer, water, plasmid storage tubes

Anion Exchange Resin
Bacteria
Cloning, PCR
10 Preps
PureLink™
10 Preps
Plasmid DNA
Spin Column
Maxi
Mammalian
Manual Protocols
> 1 mg (Large-Scale) Plasmid DNA, > 200 μg (Large-Scale) Plasmid DNA
Plasmid Purification Kit
Room Temperature
90 min.
Product Suggestions

Product Suggestions

Videos
Safety and Handling

Safety and Handling

Product Identifier
  • Lysis Buffer (L7)

Signal Word
  • Warning

Hazard Category
  • Skin corrosion/irritation Category 2
  • Serious eye damage/eye irritation Category 2

Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.

Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P264-Wash thoroughly after handling.
  • P302+P352-IF ON SKIN: Wash with plenty of water/.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P362+P364-Take off contaminated clothing and wash it before reuse.

SDS
Documents

Documents

Certificates
Special Offers

Special Offers

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For Research Use Only. Not for use in diagnostic procedures.