Invitrogen™ PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit

The PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange membrane.

Brand:  Invitrogen™ A31231

Product Code. 15768227

Description

The PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange membrane. In as little as 90 minutes, purify up to 1.5 mg of high-quality plasmid DNA that is suitable for advanced transfection applications such as transfection of sensitive cells lines or genome engineering.

Advantages of the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit include:
• Simple and fast protocol—purification typically takes 90 minutes
• High yield—isolate up to 1.5 mg of high quality plasmid DNA
• Endotoxin-free plasmid—less than 0.1 EU/μg, ideal for sensitive applications
• High purity—ultralow levels of protein, RNA, and genomic DNA contamination
• Compatibility—suitable for use with ExpiCHO and Expi293 expression systems
• Endotoxin-free components— elution buffer, water, and plasmid storage tubes that are free of endotoxin

Simple and fast protocol
Unlike other anion-exchange-based endotoxin-free plasmid purification systems, the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit utilizes centrifugation- or vacuum-assisted filtration for lysate clarification and subsequent plasmid DNA purification. There is no waiting for gravity-flow columns and the entire purification typically takes 90 minutes (including the final precipitation step).

Endotoxin-free plasmid
Purify endotoxin-free (less than 0.1 EU/μg) plasmid that is ideal for sensitive applications such as transfection of primary cells, genome engineering, and research on gene therapy or plasmid vaccines in vivo.

Compatible with ExpiCHO and Expi293 expression systems
Plasmid purified using the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit is fully compatible with the Gibco ExpiCHO and Expi293 transient mammalian expression systems.

Superior quality with endotoxin-free components
Rest assured that the kit is of high quality and has additional endotoxin-free elution buffer, water, and plasmid storage tubes for added peace of mind.

Specifications

• Content And Storage: • Resuspension buffer
  • Lysis indicator
  • RNase A
  • Lysis buffer
  • Precipitation buffer
  • Endotoxin removal buffer
  • Wash buffer
  • Elution buffer
  • TE buffer
  • Expi Maxi filtration column
  • Expi Maxi DNA binding column
  • 50 mL conical tubes
  
  Endotoxin-free components: elution buffer, water, plasmid storage tubes
  
  
• Isolation Technology: Anion Exchange Resin
• Sample Type: Bacteria
• For Use With (Application): Cloning, PCR
• No. of Reactions: 25 Preps
• Product Line: PureLink™
• Quantity: 25 Preps
• Target: Plasmid DNA

• Format: Spin Column
• Scale: Maxi
• Expression System: Mammalian
• High-throughput Compatibility: Manual Protocols
• Prep Scale: > 1 mg (Large-Scale) Plasmid DNA, > 200 μg (Large-Scale) Plasmid DNA
• Product Type: Plasmid Purification Kit
• Shipping Condition: Room Temperature
• Test Time: 90 min.

Safety and Handling

Product Identifier

• 25 Preps PURELINK ENDOFREE MAXI PLASMID

Signal Word

• Danger||Warning

Hazard Category

• LONG-TERM AQUATIC HAZARD Chronic 3
• Serious eye damage/eye irritation Category 1
• Serious eye damage/eye irritation Category 2
• Flammable liquid Category 2
• Skin corrosion/irritation Category 2

Hazard Statement

• H225-Highly flammable liquid and vapour.
• H315-Causes skin irritation.
• H318-Causes serious eye damage.
• H319-Causes serious eye irritation.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P233-Keep container tightly closed.
• P242-Use only non-sparking tools.
• P264-Wash hands thoroughly after handling.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P370+P378-In case of fire: Use dry sand, dry chemical, or alcohol resistant foam to extinguish.
• P403+P235-Store in a well-ventilated place. Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : acetic acid, poly(oxy-1,2-ethanediyl).alpha.-[4-(1,1,3,3-tetramethylbutyl)phenyl]-.omega.-hydroxy, e

For Research Use Only. Not for use in diagnostic procedures.