Invitrogen™ PureLink™ Fast Low-Endotoxin Midi Plasmid Purification Kit

The PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (<1 EU/μg) plasmid DNA in about 30 minutes.

Brand:  Invitrogen™ A36227

Product Code. 17113129

Description

The simple precipitation-free protocol yields up to 0.4 mg plasmid, suitable for standard transfections and all molecular biology applications such as cloning and sequencing.

• Ultra-fast protocol—innovative silica-based columns enable purification of transfection grade plasmid DNA in under 30 minutes
• Low endotoxin plasmid—the resulting plasmid contains less than 1 EU/μg endotoxins
• High yields—isolate up to 0.4 mg of high-quality plasmid DNA
• Colored buffers—the kit features colored buffers that permit error-free visualization of complete bacterial cell lysis and subsequent neutralization

Specifications

• Content And Storage: • Resuspension buffer, store at 4°C upon reciept
  • Lysis buffer, store at room temperature
  • Precipitation buffer, store at room temperature
  • Binding buffer, store at room temperature
  • Wash buffer 1, store at room temperature
  • Wash buffer 2 (concentrate), store at room temperature
  • Elution buffer, store at room temperature
  • Column assemblies, store at room temperature
  • Syringe filters, store at room temperature
  • Syringe plungers, store at room temperature
  • Collection tubes, store at room temperature
  
  
• Isolation Technology: Silica Column
• For Use With (Application): Cloning, PCR
• Prep Scale: Midi scale input culture of 25-100 mL to obtain yields of up to 200 μg
• Product Type: Plasmid Purification Kit
• Shipping Condition: Room Temperature

• Format: Kit
• Scale: Midi
• No. of Reactions: 50 Preps
• Product Line: PureLink™
• Quantity: 50 Preps
• Test Time: 30 min.

Safety and Handling

Product Identifier

• PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit

Signal Word

• Danger

Hazard Category

• Serious eye damage/eye irritation Category 2
• Flammable liquid Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H225-Highly flammable liquid and vapour.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H336-May cause drowsiness or dizziness.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P271-Use only outdoors or in a well-ventilated area.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P370+P378-In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
• P403+P233-Store in a well-ventilated place. Keep container tightly closed.
• P403+P235-Store in a well-ventilated place. Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Sodium hydroxide, Isopropanol

For Research Use Only. Not for use in diagnostic procedures.