Invitrogen™ PureLink™ Microbiome DNA Purification Kit

The Invitrogen™ PureLink™ Microbiome DNA Purification Kit uses proven PureLink™ spin column technology for robust yields of purified DNA ready for downstream PCR, sequencing or other applications.

Brand:  Invitrogen™ A29790

Product Code. 15449733

Description

The Invitrogen™ PureLink™ Microbiome DNA Purification Kit uses proven PureLink™ spin column technology for robust yields of purified DNA ready for downstream PCR, sequencing or other applications. The highly efficient triple lysis approach, fast removal of inhibitors, and versatility makes PureLink the ultimate kit for microbiome research projects as well as programs aimed at rapid detection of pathogenic bacteria in various samples.

• Efficient lysis of all microorganisms (including durable species with thicker and more complex cell walls) by a combination of heat, chemical, and mechanical disruption with specialized beads
• Elimination of inhibitory compounds by precipitation using a novel cleanup buffer
• Streamlined protocols for numerous biological samples
• Recovery of highly pure DNA compatible with common downstream applications such as qPCR and next-generation sequencing

Certifications

Certificates of Analysis for each lot

Services

Product performance guaranteed for 1 year from date of sale

Specifications

• Column Type: Spin Column
• Isolation Technology: Silica Spin Column
• Sample Type: Microbial Culture, Saliva, Soil, Stool, Host DNA from Stool, Swabs (Buccal, Vaginal, Skin, Rectal, or Environmental), Cells, Food and Environmental
• Final Product Type: Microbial and host DNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Shipping Condition: Room Temperature
• Yield: ≤25 μg

• Content And Storage: • 40 mL S1 Lysis Buffer; room temperature
  • 5 mL S2 Lysis Enhancer; room temperature
  • 12.5 mL S3 Cleanup Buffer; room temperature
  • 45 mL S4 Binding Buffer; room temperature
  • 13 mL S5 Wash Buffer (concentrated); room temperature
  • 5 mL S6 Elution Buffer; room temperature
  • 50 PureLink Columns with Collection Tubes; room temperature
  • 100 PureLink Collection Tubes; room temperature
  • 50 Bead Tubes; room temperature
  
  
• Scale: Mini
• Elution Volume: 50 to 200 μL
• For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
• Quantity: 50 Preps kit
• Test Time: Up to 55 min. (Including sample homogenization)
• Starting Material Amount: Buccal/Vaginal/Skin/Rectal/Environmental Swab: 1 swab
  Microbial Culture: ≤5 mL
  Saliva: ≤2 mL
  Soil: ≤2 g
  Stool: ≤2 g
  Urine: ≤10 mL

For research use only. Not for use in diagnostic procedures.