Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents

Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent.

Product Code	Brand	Product Type	Quantitation Range	Packaging Type
10545213	Invitrogen™ P7589	Quant-iT PicoGreen dsDNA Assay Kit	50 pg to 2 μg	1 x 1 mL Bottle
10398702	Invitrogen™ P11496	Quant-iT PicoGreen dsDNA Assay Kit	50 pg to 2 μg	10 x 100 μL Tubes
10535213	Invitrogen™ P7581	Quant-iT PicoGreen dsDNA Reagent	50 pg to 2 μg	1 x 1 mL Bottle
11559196	Invitrogen™ P11495	Quant-iT PicoGreen dsDNA Reagent	50 pg to 2 μg	10 x 100 μL Tubes

Description

Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Picogreen assays are compatible with most microplate readers and fluorometers.

Rapidly detect as little as 0.25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA, miniprep DNA, or PCR amplification products.

PicoGreen dsDNA quantitation reagents are
• Significantly more sensitive than UV absorbance readings, saving precious sample amounts
• Specific for dsDNA in the presence of RNA
• Easy to use—just add the dye working solution to the sample, incubate five minutes, and read
• Suitable for use in 96- and 384-well plate formats
• Compatible with most fluorescence-based microplate readers and fluorometers
• Picogreen aproximate fluorescence absorption/emission maxima is 502/523 nm, bound to dsDNA

Applications
The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral DNA quantitation.

Specifications

• Detection Method: Fluorescence
• For Use With (Equipment): Microplate Reader
• Quantitation Range: 50 pg to 2 μg
• Shipping Condition: Room Temperature

• Excitation/Emission: 500/525
• No. of Reactions: 200 Assays (2 mL assay volume)
• Product Line: PICOGREEN, Quant-iT