Rabbit anti-Goat IgG Recombinant Secondary Antibody, Alexa Fluor™ Plus 488, Invitrogen™

Rabbit Recombinant Monoclonal Secondary Antibody

Product Code	Brand	Quantity
17856472	Invitrogen A5657450UL	50 μL
17896762	Invitrogen A56574	250 μL

Description

Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity and superior lot-to-lot consistency. This recombinant antibody has been engineered with point mutations that eliminate binding to Fc receptors, enabling reduction in background and superior signal to noise. Obtain superior images with the new Invitrogen Alexa Fluor Plus secondary antibodies. Make your low abundant targets visible, minimize time spent optimizing, and make your precious samples count. Alexa Fluor Plus secondary antibodies provide brighter signal compared to leading Alexa Fluor secondary antibodies, providing you with better signal-to-noise for your critical experiments. Alexa Fluor Plus secondary antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor Plus secondary antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning images. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.

Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Goat IgG
• Concentration: 1 mg/mL
• Classification: Recombinant Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.5
• Primary or Secondary: Secondary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

• Clone: 21H44L11
• Applications: Flow Cytometry, Immunocytochemistry, Western Blot
• Conjugate: Alexa Fluor Plus 488
• Host Species: Rabbit
• Target Species: Goat
• Isotype: IgG
• Purification Method: Affinity chromatography