Thermo Scientific™ Pierce™ Rapid Gold BCA Protein Assay Kit

Thermo Scientific™ Pierce™ Rapid Gold BCA Protein Assay Kit is two-component, high-precision, detergent-compatible assay optimized to measure (at 480 nm) total protein concentration compared to protein standard curve of known concentrations.

Brand:  Thermo Scientific™ A53225

Product Code. 15776178

Description

• A fast, two-component, high-precision, detergent-compatible assay optimized to determine total protein concentration
• Can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications
• Assay maintains the key characteristics of the traditional BCA assay but allows a fast time and room temperature incubation equal to dye-binding methods
• Provides comparable accuracy but with a 5 min., room temperature incubation and measured at 480 nm
• This improvement eliminates the need to wait or expose the samples to elevated temperatures for a fast time to results
• Exhibits similar protein-to-protein variation as traditional BCA Protein Assay and less protein-to-protein variation than dye-binding protein assay methods (Bradford)
• Unaffected by typical concentrations of most ionic and non-ionic detergents
• Linear working range for BSA of 20 to 2000 μg/mL
• Store in refrigerator at 2°C to 8°C

Specifications

• Assay Range: 20 to 2000 μg/mL
• Assay: Rapid Gold BCA Assay
• Product Line: Pierce™
• Specificity: Not Target-Specific
• For Use With (Equipment): Spectrophotometer, Microplate Reader
• Shipping Condition: Wet Ice

• Content And Storage: Store in refrigerator (2–8°C).
• Detection Method: Colorimetric
• Product Type: Protein Quantitation Assay
• For Use With (Application): Solution-based Detection, Absorbance
• Quantity: 500 mL

Safety and Handling

Product Identifier

• Pierce Rapid Gold BCA Protein Assay Kit

Signal Word

• Warning

Hazard Category

• LONG-TERM AQUATIC HAZARD Chronic 2
• ACUTE AQUATIC Acute 1
• Serious eye damage/eye irritation Category 2
• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.
• H319-Causes serious eye irritation.
• H400-Very toxic to aquatic life.
• H411-Toxic to aquatic life with long lasting effects.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P233-Keep container tightly closed.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P242-Use only non-sparking tools.
• P243-Take precautionary measures against static discharge.
• P264-Wash hands thoroughly after handling.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P391-Collect spillage.
• P403+P235-Store in a well-ventilated place. Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : acetonitrile, copper(II) sulphate pentahydrate

For Research Use Only. Not for use in diagnostic procedures.