Rat anti-Mouse IgG2a (Heavy chain) Secondary Antibody, FITC, Invitrogen™

Rat Monoclonal Secondary Antibody

Brand:  Invitrogen MA516789

Product Code. 15216039

Description

For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Rat anti Mouse IgG2a:Biotin, clone LO-MG2a-7 recognizes the gamma 2a heavy chain of mouse immunoglobulin and does not cross-react with other murine immunoglobulin classes or subclasses.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Mouse IgG2a (Heavy chain)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.1% sodium azide
• Immunogen: Purified IgG from BALB/c mice
• Quantity: 500 μg
• Primary or Secondary: Secondary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
• Isotype: IgG1

• Applications: Flow Cytometry
• Clone: LO-MG2a-7
• Conjugate: FITC
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Mouse
• Form: Liquid