Invitrogen™ Rat NFKB-p105 ELISA Kit

For the quantitative detection of Rat NFKB-p105

Brand:  Invitrogen™ EEL138

Product Code. 18047454

Description

NF-kB is a nuclear transcription factor activated by various extra and intracellular stimuli such as cytokines, UV radiation, stress, in injury, and by bacterial and viral products. It is involved in regulation of various cellular events including cell growth, differentiation, proliferation, apoptosis and inflammation. NFKB1 (p50), a 50KDa functional sub-unit of NF-kB, is a member of Rel protein family. It is synthesized as a p105 precursor protein and consists of an N-terminal conserved RHD-region containing nuclear localization signal, DNA-binding and dimerization domains. NFKB1 (p50) forms homodimers or heterodimerizes with p65, forming the functional NF-kB factor. NFKB1 (p50) directs the nuclear translocation of NF-kB and is instrumental in its DNA-bidning. Pathological role of NF-kB has been suggested in AIDS, hematogenic cancer cell metastasis and rheumatoid arthritis.

Specifications

• Accession Number: Q63369
• Assay Sensitivity: 0.1 ng/mL
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: NFKB1
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Test Time: 1 hr 20 min

• Assay Range: 0.16 to 10 ng/mL
• Conjugate: HRP
• Sample Type: Serum
• Gene ID (Entrez): 81736
• Interassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Quantity: 96 Tests
• Sample Volume: 100 µL
• Target: Rat
• Total Assay Time: 3 hr 30 min