Invitrogen™ ReadyProbes™ Tissue Autofluorescence Quenching Kit

Minimizes unwanted autofluorescence in FFPE and frozen tissues due to aldehyde fixation, red blood cells, and structural elements such as collagen and elastin

Brand:  Invitrogen™ R37630

Product Code. 17162534

Description

• The ReadyProbes Tissue Autofluorescence Quenching Kit minimizes unwanted autofluorescence in FFPE and frozen tissues due to aldehyde fixation, red blood cells, and structural elements such as collagen and elastin.
• If the target signal is low enough, then this background signal can interfere with the interpretation of immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence(IF), and in situ hybridization (ISH) assays.
• The ReadyProbes Tissue Autofluorescence Quenching Kit minimizes most kinds of autofluorescence, with the exception of autofluorescence from lipofuschin granules.
• The ReadyProbes Tissue Autofluorescence Quenching Kit contains three components that are combined just before use. Combine components A, B, and C in a 1:1:1 ratio.
• Mix components A and B together before adding component C. The resulting solution is then applied to the tissue for 2–5 minutes at room temperature.
• This is done after the blocking, primary antibody, and secondary antibody steps, but before application of any counterstains like nuclear stains.
  
  

Specifications

• Quantity: 1 Kit
• Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice
• Product Type: Blocking Reagent

• Content And Storage: A kit containing 5 mL each ready-to-use (1X) solutions of reagent A, B, and C in three clear HDPE bottles.
• Store at 2–8°C.
When stored as directed, product can be used for 3 months after receipt.One kit is sufficient to tre
• Product Line: ReadyProbes™

For Research Use Only. Not for use in diagnostic procedures.