R&D Systems™ Recombinant Human BMP-4 Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Product Code	Brand	Quantity	Formulation
18176280	R&D Systems™ 314-BP-010	10 μg	Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
18157150	R&D Systems™ 314-BP-050	50 μg	Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
18196670	R&D Systems™ 314-BP-010/CF	10 μg	Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA.
18340588	R&D Systems™ 314-BP-050/CF	50 μg	Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA.
18156870	R&D Systems™ 314-BP-01M	1 mg	Lyophilized from a 0.2 um filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
18793989	R&D Systems™ 314-BP-500	500 μg	Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
18731388	R&D Systems™ 314-BP-500/CF	500 μg	Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA.

Description

Measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.70-7.00 ng/mL. Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 3.00-30.0 ng/mL.

Specifications

• Gene ID (Entrez): 652
• Source: Mouse myeloma cell line,NS0-derived human BMP-4 protein Ser293-Arg408
• Conjugate: Unconjugated

• Molecular Weight (g/mol): M.W. (Observed): 19-24 kDa, reducing conditions 35-41 kDa, non-reducing conditions
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied.